This study explored the population dynamics of the endangered perennial plant Pterygopleurum neurophyllum (Maxim.) Kitag through a multi-year winter deadbiomass removal approach. In 2022, a total population census was conducted at Wondong Wetland (Yangsan, Korea), where seven spatially independent patches were identified (P-1 to P-7). From 2023 to 2025, winter dead-biomass removal was implemented in two of these patches (P-1 and P-2), while the remaining patches served as controls. Annual population counts were monitored, and the effects of winter dead-biomass removal over time were analyzed using a generalized linear mixed model (GLMM) with a Poisson distribution. The interaction between management and year was highly significant (Wald χ 2=103.93, p<0.001), indicating differing temporal trajectories between the removal and control populations. No significant management effect was observed in 2023; however, the removal plots demonstrated a dramatic increase in 2024 (EMMs: Control=5.05 vs. Removal=92.77), which continued in 2025 (3.89 vs. 104.99). These findings indicate that winter dead-biomass removal significantly promotes the population expansion of P. neurophyllum, with ecological responses emerging cumulatively rather than immediately. This study provides empirical evidence that repeated winter dead-biomass removal, rather than a single-year disturbance, is crucial for the restoration and conservation of endangered wetland plant species.