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시험관 내 큰잎알쏭이모자반 추출물의 DNA 손상 및 세포 사멸 유도를 통한 구강 편평세포 암종의 성장 억제 능력 KCI 등재
Sargassum Pallidum Extract Inhibits OSCC Growth through DNA Damage and Apoptosis Induction in Vitro
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/447625
pp.135-142
This study investigated the anticancer efficacy and underlying mechanism of Sargassum pallidum (S. pallidum) extract and evaluated its potential as a natural therapeutic candidate against oral squamous cell carcinoma (OSCC). To assess its cytotoxic and antiproliferative effects, human OSCC cell lines were treated with varying concentrations of S. pallidum extract. The extract significantly inhibited cell viability and proliferation in a dose-dependent manner. Moreover, treatment with S. pallidum extract induced DNA damage, evidenced by increased γH2AX protein expression and nuclear foci formation. This DNA damage subsequently triggered apoptosis, as indicated by increased levels of the proapoptotic protein Bak and decreased levels of the antiapoptotic protein BCL-2. Furthermore, terminal deoxynucleotidyl transferase dUTP nick-end labeling assay results confirmed apoptotic DNA fragmentation following treatment. Collectively, these findings demonstrate that S. pallidum extract suppresses OSCC cell viability and proliferation by inducing DNA damage–mediated apoptosis, highlighting its potential as a natural anticancer agent in OSCC therapy.
Ⅰ. INTRODUCTION
Ⅱ. MATERIALS and METHODS
1. Cell lines and cell culture
2. Quantitative real-time polymerase chainreaction (qRT-PCR)
3. Western blot assay
4. Immunofluorescence
5. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay
6. 5-Ethynyl-2′-deoxyuridine (EdU) assay
7. Terminal deoxynucleotidyl transferase dUTPnick-end labeling (TUNEL) assay
Ⅲ. RESULTS
1. S. pallidum extract inhibited cell viability andproliferation in HSC-2 cells
2. S. pallidum extract induced DNA damage inHSC-2 cells
3. S. pallidum extract promoted apoptosis inHSC-2 cells
Ⅳ. DISCUSSION
ACKNOWLEDGMENTS
REFERENCES
