Comparative Analysis of Structural, Thermal, Hydration, and Functional Characteristics of Black Soybean (Glycine max (L.) Merr.) Cultivars
This study examined the structural, physicochemical, and functional characteristics of five black soybean (Glycine max (L.) Merr.) cultivars—Cheongja5, Danheuk, Socheongja, Seum, and Soriheuk—bred and cultivated in Korea. We conducted comprehensive analyses on morphology, microstructure, thermal and hydration properties, pasting behavior, and antioxidant activity to identify cultivar-specific differences in processing suitability and functional properties. The results indicated significant varietal variations in seed coat ratio, cotyledon density, and color, which affected thermal stability, hydration, and viscosity development during heating. Cultivars with compact cotyledon matrices, such as Cheongja5 and Danheuk, displayed high enthalpy (ΔH), low solubility, and limited viscosity development, suggesting high structural stability and low thermal reactivity-traits favorable for thermally stable or beverage-type applications. In contrast, cultivars with looser structures and greater surface exposure, like Seum and Socheongja, exhibited higher swelling power, dispersion stability, and RVA viscosities, indicating their suitability for viscous or semi-solid systems. Notably, Soriheuk showed the highest antioxidant activity and levels of phenolic and flavonoid compounds, correlating with its high seed coat ratio and dark pigmentation, positioning it as a promising functional ingredient. These findings underscore that the physicochemical and structural diversity among black soybean cultivars significantly influences their processing performance and functional potential, providing a scientific foundation for selecting and developing functional cultivars.