Lactobacillus johnsonii JERA01-supplemented feed additive (Lj-A) was produced by fermenting dried porcine blood with Lactobacillus johnsonii (Lj). Lj-A has highly digestible nutrients, bioactive peptides, and probiotic effects. To assess the immunomodulatory potential of Lj-A, it was tested on splenocytes of C57BL/6 mouse. Lj-A was treated on splenocytes in a range of concentration, 0-100 μg/ml. The metabolic activity of splenocytes was enhanced by Lj-A, as shown in MTT assay. Also, some splenocyte clusters were observed under a bright-field microscope on the wells treated with Lj-A. The splenocyte clusters indicated that the cells were activated and proliferating in response to Lj-A. These findings suggest that Lj-A stimulates splenocytes to promote immune cell activation, as evidenced by increased tumor necrosis factor-alpha and interleukin-12 production, thereby enhancing immunological defense functions. In vitro treatment of splenocytes with Lj-A increased the proportions of T cells, B cells, and CD25+ cells. In vivo, immune cell activity was evaluated in C57BL/6 mice orally administered with Lj-A at a dose of 100 mg/day. The proportion of dendritic cells in peritoneal cells was increased along with elevated CD54+ expression. Additionally, the proportions of B cells, CD25+ cells in Peyer’s patch cells increased as well. These results suggest that Lj-A may contribute to the enhancement of immune function and the maintenance of long-term health in animals.

Abstract
서 론
재료 및 방법
    실험동물과 시약
    비장세포의 분리와 물질 처리
    비장세포의 대사활성도 측정
    세포 형태 관찰
    사이토카인 생산량 측정
    마우스 경구투여
    유세포 분석
    통계분석
결 과
    비장세포에서 Lj-A 처리 후 대사활성도 증가
    비장세포 형태 관찰
    Lj-A에 의한 사이토카인 생산
    Lj-A에 의한 비장세포 활성화 마커 발현
    Lj-A를 경구투여 후, 골수성 유래(Myeloid origin) 세포의활성화 분석
    Lj-A를 경구투여 후, 림프성 유래 (Lymphoid origin)세포의 활성화 분석
고 찰
감사의 글
REFERENCES

• Dong-Ju Lee(College of Veterinary Medicine, Jeju National University, Jeju 63243, Korea)
• Hong-Gu Joo(College of Veterinary Medicine, Jeju National University, Jeju 63243, Korea, Veterinary Medical Research Institute, Jeju National University, Jeju 63243, Korea) Corresponding author