Mitochondrial genomes have been documented for select subspecies of Rhinolophus ferrumequinum (the greater horseshoe bat); however, a lack of comprehensive population-level sequences continues to limit our understanding of its complex phylogeographic history. This study reports the complete mitochondrial genome of the R. ferrumequinum sampled from South Korea and sequenced at Hoseo University. The circular mitogenome is 16,795 bp in length and comprises the typical 37 mitochondrial genes (13 protein-coding genes, 22 tRNA genes, two rRNA genes) and one non-coding control region, showing an overall base composition of GC content 42.74%. Phylogenetic analysis based on mitochondrial protein‑coding genes places this genome in R. ferrumequinum providing a new genomic resource for population genetics of horseshoe bats in East Asia.

Abstract
INTRODUCTION
MATERIALS AND METHODS
    Study animals and specimen collection
    DNA extraction
    Library preparation and sequencing
    Bioinformatics
RESULTS
    Mitogenomic characteristics
    Phylogenetic analysis
DISCUSSION
ACKNOWLEDGEMENTS
DATA AVAILABILITY STATEMENT
CONFLICT OF INTEREST
REFERENCES

• Chunghyun Kim(Hoseo Bat Research Center, Hoseo University, Asan, Chungnam 31499, Korea)
• Dahyun Hwang(Hoseo Bat Research Center, Hoseo University, Asan, Chungnam 31499, Korea, Department of Biomedical Laboratory Science, College of Biohealth Sciences, Hoseo University, Asan, Chungnam 31499, Korea)
• Hyun-Seok Jin(Hoseo Bat Research Center, Hoseo University, Asan, Chungnam 31499, Korea, Department of Biomedical Laboratory Science, College of Biohealth Sciences, Hoseo University, Asan, Chungnam 31499, Korea)
• Dongju Jung(Hoseo Bat Research Center, Hoseo University, Asan, Chungnam 31499, Korea, Department of Biomedical Laboratory Science, College of Biohealth Sciences, Hoseo University, Asan, Chungnam 31499, Korea)
• Sangjung Park(Hoseo Bat Research Center, Hoseo University, Asan, Chungnam 31499, Korea, Department of Biomedical Laboratory Science, College of Biohealth Sciences, Hoseo University, Asan, Chungnam 31499, Korea)
• Sang-Hee Jeong(Hoseo Bat Research Center, Hoseo University, Asan, Chungnam 31499, Korea, Department of Biomedical Laboratory Science, College of Biohealth Sciences, Hoseo University, Asan, Chungnam 31499, Korea) Corresponding author