Uterine adenocarcinoma is the most common neoplasm in companion rabbits. A 5-year-old intact female Lionhead rabbit was presented with a 5-day history of hematuria, hyporexia, and decreased fecal output and pellet size. Thoracic and abdominal radiography, ultrasonography, and hematologic analyses revealed enlarged uterine masses in the caudoventral abdomen. An ovariohysterectomy was performed, and histopathology confirmed uterine adenocarcinoma. The rabbit recovered postoperatively and has remained clinically well 14 months after surgery. This case report describes the successful surgical management of uterine adenocarcinoma in a companion rabbit by ovariohysterectomy.