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pp.151-159
This study evaluated the safety of a water extract prepared from the roots of Asparagus officinalis L. (asparagus root extract, ARE) for its potential use as a food or functional material. Acute oral toxicity and a standard genotoxicity battery were conducted according to internationally accepted guidelines. In the acute oral toxicity study, female Sprague-Dawley rats received a single oral dose of ARE at 300 or 2,000 mg/kg body weight. No treatment-related mortality, clinical signs, suppression of body weight gain, or gross pathological findings were observed. In the bacterial reverse mutation assay, ARE did not induce any biologically relevant increase in revertant colonies in histidine-requiring Salmonella typhimurium tester strains (TA98, TA100, TA1535, and TA1537) or in Escherichia coli WP2uvrA, up to 5,000 μg/plate, with or without metabolic activation. In addition, ARE did not increase the number of structural or numerical chromosomal aberrations in Chinese hamster lung (CHL/IU) cells or the frequency of micronucleated polychromatic erythrocytes in male Institute of Cancer Research (ICR) mice, and no bone marrow toxicity was observed. These findings suggest that ARE showed low toxicity under the tested conditions and was not associated with acute oral toxicity or genotoxicity under the present experimental conditions.
I. Introduction
II. Materials and Methods
1. Preparation of asparagus root extract
2. Acute oral toxicity study
3. Bacterial reverse mutation assay
4. In vitro mammalian chromosomal aberration test
5. In vivo micronucleus test
6. Statistical analysis
III. Results and Discussion
1. Acute oral toxicity
2. Bacterial reverse mutation assay
3. In vitro chromosomal aberration assay
4. In vivo micronucleus assay
IV. Summary and Conclusion
Author biography
Conflict of Interest
References
