β-Glucan is a biologically active polysaccharide widely found in fungi, yeast, cereals, and algae, attracting attention for its immunomodulatory, antitumor, antioxidant, and metabolic regulatory effects. However, its extraction efficiency and physicochemical properties vary significantly depending on its source and structural characteristics, highlighting the need for efficient and standardized extraction processes for industrial applications. This review categorizes β-glucan extraction methods into four groups based on technological advancement and operational principles: conventional, assisted, advanced, and emerging methods. Conventional methods (hot-water, alkaline, and acid extraction) are widely used due to their simplicity and scalability but are limited by low efficiency and structural degradation. Assisted methods, including ultrasound, microwave, and enzyme-assisted extraction, improve extraction efficiency and reduce processing time. Advanced techniques such as supercritical fluid and subcritical water extraction enhance selectivity while preserving functional properties, whereas emerging methods, including deep eutectic solvents and pulsed electric fields, offer environmentally friendly alternatives. Sparassis crispa, containing over 40% β-glucan (dry basis), is a promising resource due to its highly branched β-(1→3)/(1→6) structure and strong biological activity. However, its high viscosity and gel-forming properties pose challenges in extraction and processing. Future research should focus on optimized extraction and purification processes and standardized quality evaluation systems for effective utilization.