This study analyzed the quality characteristics of 12 indigenous soybean lines cultivated in Goesan, Chungbuk province, focusing on their proximate composition, antioxidant activities, and isoflavone content. Proximate composition varied significantly among lines: moisture (4.92-12.87%), crude ash (3.79-5.98%), crude protein (15.24-39.60%), crude lipid (1.08-30.40%), and carbohydrate (34.31-65.43%). Jinunikong exhibited the highest crude protein content (39.60%), while Hayanultarikong had the lowest (15.24%). Ajukkaribamkong (brown) contained the highest crude lipid content (30.40%). Napdegikong demonstrated the highest antioxidant activities, with peak values in total polyphenol (843.79 mg%), total flavonoid (508.98 mg%), 2,2'-azino-bis (3-ethylbenzthiazoline-6- sulfonic acid) (ABTS) radical scavenging (97.20%), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging (85.92%). In contrast, Hayanultarikong and Ajukkaribamkong (brown) showed the lowest antioxidant activities, with no significant difference between them. Malonyl daidzin and malonyl genistin were the predominant isoflavones. Namdojangkong had the highest levels of these compounds (92.80 and 179.85 mg/100g, respectively). However, no isoflavones were detected in Hayanultarikong, Ajukkaribamkong (brown), and Eollukgangnangkong. Correlation analysis revealed positive relationships among crude protein, antioxidant activities, polyphenols, flavonoids, and isoflavones, while negative correlations were observed with carbohydrate content. These findings provide fundamental data on the physicochemical and functional properties of Chungbuk indigenous soybeans, confirming their potential as valuable genetic resources for functional food development and cultivar improvement.