This study aimed to evaluate the anti-inflammatory effects of pork-derived digestive hydrolysates and explore their underlying mechanisms. Pork meat was subjected to simulated gastrointestinal digestion and fractionated into different molecular weight fractions. Cytotoxicity assessment using RAW264.7 macrophages revealed no significant cytotoxic effects in any of the treatment groups. Treatment with pork hydrolysates significantly reduced the production of nitric oxide (NO) and pro-inflammatory cytokines, including tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β), in a dose-dependent manner. In addition, both mRNA and protein expression levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were markedly suppressed, with the <5 kDa fraction showing the most pronounced effects. Furthermore, the hydrolysates significantly inhibited the phosphorylation of mitogen-activated protein kinases (MAPKs), specifically c-Jun N-terminal kinase (JNK) and p38, indicating the regulation of inflammatory signaling pathways. Taken together, these results suggest that pork-derived digestive hydrolysates, particularly the low-molecular-weight fraction (<5 kDa), may exhibit anti-inflammatory effects by suppressing MAPK signaling pathways and downstream inflammatory mediators. These findings suggest that pork-derived hydrolysates, particularly the low-molecular-weight fraction (<5 kDa), may possess in vitro anti-inflammatory potential through the modulation of inflammatory signaling pathways.

Abstract
서 론
재료 및 방법
    1. 돈육 뒷다리 가수분해물 제조
    2. 세포 배양
    3. 세포생존율 측정
    4. NO 생성 억제량 측정
    5. Quantitative real-time PCR(qRT-PCR)
    6. Western blot analysis
    7. 전염증성 사이토카인 분비량 측정
    8. 통계 분석
결과 및 고찰
    1. 세포독성평가
    2. iNOS 및 COX-2 mRNA 발현 억제 효과
    3. NO 생성 억제 효과
    4. 전염증성 사이토카인(TNF-α, IL-6, IL-1β) 분비 억제효과
    5. iNOS 및 COX-2 단백질 발현 억제 효과
    6. MAPK signaling pathway 관련 단백질 발현(p-JNK,p-p38) 억제 효과
요약 및 결론
감사의 글
References

• 배인선(농촌진흥청 국립축산과학원 축산푸드테크과 농업연구사) | In-Seon Bae (Researcher, Animal Resources Food Tech Division, National Institute of Animal Science, Rural Development Administration, Wanjugun 55365, Korea) Corresponding author
• 유자연(농촌진흥청 국립축산과학원 축산푸드테크과 농업연구사) | Jayeon Yoo (Researcher, Animal Resources Food Tech Division, National Institute of Animal Science, Rural Development Administration, Wanjugun 55365, Korea)
• 박원서(농촌진흥청 국립축산과학원 축산푸드테크과 농업연구관) | Won-Seo Park (Senior Researcher, Animal Resources Food Tech Division, National Institute of Animal Science, Rural Development Administration, Wanjugun 55365, Korea)
• 함준상(농촌진흥청 국립축산과학원 축산푸드테크과 농업연구관) | Jun-Sang Ham (Senior Researcher, Animal Resources Food Tech Division, National Institute of Animal Science, Rural Development Administration, Wanjugun 55365, Korea)
• 이상엽(농촌진흥청 국립축산과학원 동물복지과 농업연구사) | Sang-Yeob Lee (Researcher, Animal Welfare Division, National Institute of Animal Science, Rural Development Administration, Wanjugun 55365, Korea)