This study aimed to comparatively evaluate the physicochemical and functional properties of Aspergillus oryzae and Rhizopus oryzae as potential alternative protein sources. Crude protein content, microstructure, pH, color, antioxidant activity, and emulsifying properties were analyzed. The crude protein content of A. oryzae was significantly higher than that of R. oryzae after freeze-drying (p<0.05), and like that of commercially available freeze-dried Fusarium venenatum. Optical microscopy revealed intertwined thin fibrous networks in both strains, regardless of freeze-drying, suggesting a meat-like structure. The pH of A. oryzae was higher than that of R. oryzae both before and after freeze-drying, and both mycelia showed significantly lower pH values after freeze-drying (p<0.05). For color, only the L* (lightness) value increased significantly after freeze-drying in both strains (p<0.05). In antioxidant assays, R. oryzae showed significantly higher DPPH radical-scavenging activity and reducing power than A. oryzae (p<0.05). For emulsifying properties, A. oryzae showed a higher emulsifying activity index (EAI), whereas R. oryzae showed a higher emulsifying stability index (ESI). These results suggest that A. oryzae and R. oryzae have potential as alternative protein sources and provide fundamental information for future mycoprotein applications.

Abstract
서 론
재료 및 방법
    1. 곰팡이 배양 및 균사체 제조
    2. 조단백질 함량 측정
    3. 광학현미경 관찰
    4. pH 측정
    5. 색도 측정
    6. DPPH 라디칼 소거능 측정
    7. Ferric reducing antioxidant power(FRAP) 측정
    8. 유화 특성 측정
    9. 통계 분석
결과 및 고찰
    1. 조단백질 함량 분석
    2. 광학현미경을 이용한 미세구조 관찰
    3. pH 분석
    4. 색도 측정
    5. 항산화 활성(DPPH 라디칼 소거능 및 FRAP)
    6. 유화 특성 측정
요약 및 결론
References

• 김주민(충남대학교 축산학과 대학원생) | Jumin Kim (Master’s Student, Dept. of Animal Science, Chungnam National University, Daejeon 34134, Korea)
• 조현욱(충남대학교 축산학과 대학원생) | Hyun Uk Cho (Master’s Student, Dept. of Animal Science, Chungnam National University, Daejeon 34134, Korea)
• 남채민(충남대학교 축산학과 대학원생) | Chemin Nam (Master’s Student, Dept. of Animal Science, Chungnam National University, Daejeon 34134, Korea)
• 전서현(충남대학교 축산학과 대학원생) | Seohyeon Jeon (Master’s Student, Dept. of Animal Science, Chungnam National University, Daejeon 34134, Korea)
• 용해인(충남대학교 동물자원생명과학과 교수, 충남대학교 축산학과 교수) | Hae In Yong (Professor, Dept. of Animal Science and Biotechnology, Chungnam National University, Daejeon 34134, Korea, Professor, Dept. of Animal Science, Chungnam National University, Daejeon 34134, Korea) Corresponding author