This study evaluated the bactericidal efficacy of a Bacillus complex composed of Bacillus subtilis YBK-70, Bacillus atrophaeus YBK-W4, and Bacillus circulans YBK-04 (1:1:1, v/v/v) against Actinobacillus pleuropneumoniae (App). A bactericidal efficacy test using the broth dilution method was performed to determine the lowest effective dilution of the Bacillus complex culture medium (BCCM) (4 × 10⁶ colony-forming units/mL) after exposure to the test bacteria for 30 min at 4°C. The BCCM and test bacteria were diluted in suspensions containing low and high levels of organic matter (OM), depending on the treatment conditions. Under low OM conditions, the bactericidal activity of the BCCM against App was observed at all 5.0-fold dilutions. Under high OM conditions, the bactericidal activity of the BCCM against App was observed at all 1.25-fold dilutions. The recommended dilution ratios of the BCCM under low and high OM conditions were 4.0-fold and 1.0-fold, respectively. As the BCCM exhibits bactericidal efficacy against App, it may serve as an effective agent for limiting the spread of bacterial diseases in animals.

Abstract
서 론
재료 및 방법
    복합미생물 조성
    공시 균주 및 배양
    복합미생물 희석
    복합미생물과 App 반응
    중화반응 및 App 증식여부 판정
    복합미생물 유효용량 및 권장용량 설정
결 과
    복합미생물 희석액의 App에 대한 효력
    복합미생물의 유효용량 및 권장용량
고 찰
REFERENCES

• Sung Bum Lee(Yessbio Co. Ltd., Gwangju 61005, Korea)
• Ik Ho Choi(Yessbio Co. Ltd., Gwangju 61005, Korea)
• Youn-Tae Chi(Yessbio Co. Ltd., Gwangju 61005, Korea)
• Lahyun Park(College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea)
• Hu-Jang Lee(College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea) Corresponding author
• Jin-Kyung Kim(Department of Dental Hygiene, Daegu Health College, Daegu 41453, Korea) Corresponding author