논문 상세보기
pp.43-50
정자의 동결보존을 위한 새로운 기술개발 목적은 동결과정에서 최소한의 손상으로, 응해 후 최대한 높은 활력도의 정자를 얻는 것이다 정자가 난자와 수정하기 위해서는 적당한 생존성과 운동성을 유지해야 하는데, 가장 일반적인 방법으로는 정자의 진진 운동성과 첨체의 정상 여부 및 형태 검사방법 등이 있다 본 연구는 사람 정액을 동결보존 할 때 semi-programmable freezer를 이용한 완만동결 방법과, 액체질소의 vapor를 이용한 급속동결 방법이
The objective of this study was to investigate the effect of cryopreservation by slow and rapid freezing on the sperm motility index, viability and morphology of post-thaw human spermatozoa. After rapid freezing and thawing, sperm motility index was significantly higher (MOT:47.4020.06%, VCL : 38.1215.58 m/s, VSL : 28.1914.10 m/s, VAP:33.6415.15 m/s, and HYP 2.772.71%) than slow freezing and thawing(MOT : 43.39 18.79%, VCL .33.91 13.50 Um/s, VSL . 19.980.88 m/s, VAP : 24.6011.72 m/s, and HYP . 1.331.57% ; P<0.05). But sperm Linearity(LIN) was significantly lower(28.83 10.35) comparing to the slow freezing method(34.64 11.36 ; P<0.05). On the other hand, significant difference were not observed MAD, WOB, DNC and DNM by slow and rapid frozen-thawed methods. After rapid freezing and thawing, sperm viability was lower(60 2.2%) than slow freezing method(62 2.1%) and sperm morphology was higher(467.7%) than that(44: 8.3). But there was no significantly These results indicate that rapid freezing method was positive effect of sperm cryopreservation in human.
