본 실험은 돼지정액의 동결보존을 위한 희석액과 냉각속도 및 동해방지제의 적정농도를 결정하기 위해 실시하였으며, 얻어진 결과는 다음과 같다. 1. 에서 까지의 냉각속도에서는 LEY 희석액에서 분당 로 냉각하는 것이 생존율과 정상 첨체율에서 가장 높은 결과를 얻었다. 2. LEY 희석액이 BF5와 M-Soejima 희석액보다 정자를 동해로부터 보호하는 능력이 우수하였다. 3. LEY 희석액에 첨가하는 glycerol의 농도는 3 또는 가 의 glycerol

This experiment was carried out to investigate the extender, cooling rate and concentration of glycerol for freezing of boar semen. The result obtained were summarized as follows: 1. Optimal cooling rate was from 25 to in LEY extender on the viability and normal acrosome after thawed. 2. The LEY extender was effective in protecting frozen boar semen from cold shock among the extenders(p<0.001, respectively). 3. The sperm viability and normal acrosome rates after thawing was showed greater in the 3 or of glycerol concentration than in LEY extender. 4. Viability of sperm was higher when both 15mM of fructose and 3 or glycerol were added to the LEY extender compared with other concentrations of fructose and glycerol were added it(p<0.001).

• 심금섭(천안연암대학)
• 김광식(천안연암대학)
• 서경덕(천안연암대학)
• 송해범(대구대학교)