논문 상세보기
pp.263-271
본 연구는 미니 돼지와 일반 돼지(Duroc)의 동결융해 후 정자의 수정 능력을 비교하여 동결 보존체계의 기틀을 확립하고자 하였다. 정액 제조는 수압법으로 정액 채취하여 1차 희석하였다. 동결은 LEY (1차: 11% -lactose+egg yolk, 2차: 1차 동결액+glycerol+OEP) 동결액을 이용하여 동결을 실시하여 동결 보존하였다. 동결 정액의 융해는 0.5 ml straw를 각각 20, 37 및 water bath에서 1분, 40 초 및
The purpose of this study was undertaken to compare ability of frozen-thawed sperm characteristics between two strains (miniature pig and Duroc). The semen was collected by gloved-hand method into a pre-warmed () thermos bottle. The semen was diluted with same volume extender and added to LEY solution for freezing. The diluted semen was placed in 0.5 ml straws, and freezing was initiated by exposing the straws to liquid nitrogen () vapours for 10 min before placing them into for cryopreservation. The frozen-semen straw were thawed at 20, 37 and for 1 min, 45 sec and 10 sec within water-bath. The semen sample were evaluated at 0, 3, 6, 9, and 12 h after incubation at for analysis of sperm ability. Abnormality of spermatozoa in miniature pig was significantly (p<0.05) higher than that in Duroc at 0, 9 and 12 h of post-thawing incubation after frozen-thawing. The percentage of F-patterned spermatozoa in miniature pig was significantly (p<0.05) lower, while the percentage of AR (acrosome reacted spermatozoa) pattern was higher in the miniature than in the Duroc. On the other hand, there was no significant difference in the viability of spermatozoa thawed at different temperature () between two species, but the viability in miniature pig was higher (p<0.05) than in Duroc when sperm was thawed at . In conclusion, this study suggest that suitable freezing method for miniature pig semen is required for increasing post-thawing viability and fertilization capacity.
