논문 상세보기

Developmental Potential of Bovine Nuclear Transfer Embryos Cultured in Serum-free Medium

  • 언어ENG
  • URLhttps://db.koreascholar.com/Article/Detail/61979
구독 기관 인증 시 무료 이용이 가능합니다. 4,000원
한국동물번식학회 (The Korean Society of Animal Reproduction)
초록

The purpose of this study was to investigate the development of bovine nuclear transfer (NT) embryos cultured in serum-free conditions. Bovine NT embryos cultured in various culture conditions were compared blastocyst development, total cell number and apoptosis using TUNEL assay. In experiment 1, blastocyst rates of NT embryos were significantly higher (P<0.01) in FBS (22.0%) and BSA (26.6%) groups than in PVA (6.3%) group. Total cell number was significantly higher in FBS (78.4±19.4) and BSA (90.9±29.1) groups than in PVA group (46.0±0.0). Apoptotic cell number was significantly fewer in FBS (3.1±1.4) and BSA (1.7±1.4) groups than in PVA group (7.0±20.0) However, all of results were not different between the FBS and BSA group. In experiment 2, blastocyst rates of NT embryos were significantly higher (P<0.05) in fatty acid free-BSA (FAF-BSA) group (26.8%) than in fraction V-BSA group (11.2%). Total cell number were somewhat higher in FAF-BSA group (89.8±30.7) than in fraction V-BSA group (88.1±19.3). Apoptotic cell number were somewhat fewer in FAF-BSA (1.7±1.5) group than in fraction V-BSA group (4.2±2.9). These findings suggest that serum free condition were effective for the in vitro development of bovine NT embryos. Therefore, we concluded that fatty acid free-BSA has beneficial effect in development bovine NT embryos and can be use as a serum substitute.

저자
  • Lee, S. K(Animal Biotechnology Division, National Livestock Research Institute. RDA., Animal Resources Research Center, Konkuk University)
  • D. H. Kim(Animal Biotechnology Division, National Livestock Research Institute. RDA.)
  • G. S. Im(Animal Biotechnology Division, National Livestock Research Institute. RDA.)
  • B. C. Yang(Animal Biotechnology Division, National Livestock Research Institute. RDA.)
  • H. S. Park(Animal Biotechnology Division, National Livestock Research Institute. RDA.)
  • W. K. Chang(Animal Biotechnology Division, National Livestock Research Institute. RDA.)
  • H. T. Lee(Animal Resources Research Center, Konkuk University)