간행물
Reproductive & developmental biology
- 발행기관 한국동물번식학회
- 자료유형 학술지
- 간기 계간
- ISSN 1738-2432 (Print)2288-0151 (Online)
- 수록기간 1977 ~ 2018
- 주제분류 농수해양 > 축산학 농수해양 분류의 다른 간행물
- 십진분류KDC 527DDC 636
권호리스트/논문검색
Volume 27 No 4 (2003년 12월) 10건
1.
2003.12
구독 인증기관 무료, 개인회원 유료
This study was carried out to investigate the effect of cysteamine addition during in vitro maturation, fertilization and culture of porcine oocytes. Oocytes were matured for the first 22 h in mTCM -199 media supplemented with or without 150 μM cysteamine. They then were matured for an additional 22 h in mTCM-199 media without hormones supplemented with or without 150 μM cysteamine. When cumulus-oocyte complexes (COCs) were matured in the mTCM-199 media supplemented with cysteamine, the rates of GVBD and maturation (metaphase Ⅱ) were enhanced as compared to the media without the addition of cysteamine. Also, when COCs were matured in the mTCM-199 media supplemented with cysteamine, the rates of sperm penetration, male pronucleus formation, cleavage and blastocyst formation after in vitro fertilization were enhanced as compared to the media without the addition of cysteamine. In conclusion, it was suggested that oocytes matured for the first 22 h in mTCM-199 media supplemented with 150 μM cysteamine increased the rates of metaphase Ⅱ, sperm penetration, male pronucleus and blastocyst formation were higher as compared to the media without addition of cysteamine.
4,000원
2.
2003.12
구독 인증기관 무료, 개인회원 유료
This study was carried out to find out the changes on serum concentrations of estradiol-17β, progesterone in primiparous Duroc, Landrace and Yorkshire sows weaned at 7 or 21 days. Also, we compared the litter size at birth and weaning among the breeds weaned after lactation for 7 or 21 days. The estradiol-17β concentrations among the breeds were 6.9∼8.8 pg/ml and 6.4∼8.8 pg/ml after lactation for 7 or 21 days, respectively. The progesterone concentrations ranged from 0.3 ng/ml to 1.6 ng/ml. Duroc sow showed higher progesterone concentration compared with Landrace and Yorkshire sows weaned after lactation for 7 or 21 days. Also, we found out that litter size at birth and weaning, respectively, did not show any differences between day 7 and day 21 of lactation. From the facts mentioned above, it was suggested that very early weaning systems could work with no apparent adverse effect on prolificacy.
4,000원
3.
2003.12
구독 인증기관 무료, 개인회원 유료
The aim of this review is to give insight into the reproduction potential of the Korean native goat(KNG) doe. The mean age of the first estrus in the KNG doe is 141.24±18.l7 days. The length of the estrous cycle was recorded as being 20.58±2.63 days, with the mean duration of estrous period being 17.8±7.3 to 32.9±1.2 h, and the duration of the post-partum anestrous period being 13.4(9 to 18) to 30.1±3.8 days in the KNG doe. The ages at first delivery are 10 to 12 months(56.3％) in the KNG doe. The KNG does are no restricted breeding season, because estrus and kiddings are observed throughout the year. The mean gestation period of the KNG doe is recorded as being 150.69±6.14 days with parities having no significant effect on gestation length. The mean interval between parturitions in the KNG doe is 207.78±1.72 days with parities and birth type having no significant effect on kidding intervals. The mean litter sizes at birth in the KNG doe are 1.69±0.03 heads, and litter size at birth was affected (P<0.05) by parity. The mean birth weight of kid in the KNG is 2.04±0.30 kg with a variety as being 2.28±0.26, 2.11± 0.30 and 1.64±0.19 kg for singles, twins and triplets over of birth type, respectively. The mean mortality of 635 kids in the KNG is 23±1 ％ with a variety as being 28±3, 21±2, 16±3 and 46±15 ％ for singles, twins, triplets and quadruplets of birth type, respectively.
4,200원
4.
2003.12
구독 인증기관 무료, 개인회원 유료
Lee, H. G, P. Y. Lee, Y. K. Lee, S. J. Kim, H. K. Chung, M. K. Seo, J. K. Park, K. S. Min, W. K. Chang
The effects of additions/deletions in glycosylated residues of recombinant human EPO (rhEPO) produced in CHO-K1 on their secretion were examined. hEPO cDNA was amplified from human liver mRNA and cloned into the pCR2.1 TOPO. Using overlapping-extension site-directed mutagenesis method, glycosylation sites at 24th, 38th, 83rd, and 126th were respectively or accumulatively removed by substituting its asparagine (or serine) with glutamine. To add novel glycosylation sites, 69 and 105th leucine was mutated to asparagine. Mutant and wild type rhEPO constructs were cloned into the pcDNA3 expression vector with CMV promoter and transfected into CHO cell line, CHO-K1, to produce mutant rhEPO mutant rhEPO proteins. Enzyme-linked immunosorbant assay (ELISA) and Western analysis with monoclonal anti-EPO antibody were performed using supernatants of the cultures showing transient and stable expressions respectively. Addition of novel glycosylation reduced rhEPO secretion dramatically while deletion mutants had little effect except some double deletion mutants (△24/83 and △38/83) and triple mutant (△24/38/83). This fact suggests that not single but combination of changes in glycosyl groups affect secretion of rhEPO in cell culture, possibly via changes in their conformations.
4,000원
5.
2003.12
구독 인증기관 무료, 개인회원 유료
DNA methylation at CpG sites, which is a epigenetic modification, is associated with gene expression without change of DNA sequences. During early mouse embryogenesis, dynamic changes of DNA methylation occur. In this study, DNA methylation patterns of porcine embryos produced in vivo and in vitro were examined at various developmental stages by the immunocytochemical staining method. Interestingly, active demethylation was not observed on the paternal pronucleus of porcine zygotes. However, differences were detected in the passive demethylation process between in vivo and in vitro embryos. There was no change in the DNA methylation state until the blastocyst stage of in vivo embryos, whereas partial demethylation was observed in several blastomeres from a 4 cell stage to a morula stage of in vitro embryos. The whole genome of inner cell mass (ICM) and trophectoderm (TE) cells in porcine blastocysts were evenly methylated without de novo methylation. Our findings demonstrate that genome-wide demethylation does not occur in pig embryos during preimplantation development unlike murine and bovine embryos. It indicates that the machinery regulating epigenetic reprogramming may be different between species.
4,000원
6.
2003.12
구독 인증기관 무료, 개인회원 유료
Park, J. K, I. S. Jeon, Y. K. Lee, P. Y. Lee, S. W. Kim, S. J. Kim, H. G. Lee, J. H. Han, C. G. Park
The present study were performed to analysis the hematocrit and the red blood cells content into the blood plasma of the transgenic pigs harboring recombinent human erythropoietin gene (rhEPO). Mouse whey acidic protein (mWAP) linked to rhEPO gene was microinjected into pronuclei of porcine one-cell zygotes. After delivered of offspring, PCR analyses identified one mWAP-rhEPO transgenic founder offspring(F/sub 0/). The first generation of transgenic pig (F/sub 0/) harboring mWAP-hEPO appeared to be a male, and the second generation (F₁) pigs were made by natural mating of F/sub 0/ with domestic swine, and male and female transgenic pigs (F₁) were identified by PCR. The blood samples from transgenic and normal pigs were collected for 50 days during lactation and were counted the red blood cell (RBC) numbers and Hematocrit (HCT) content into the blood. The transgenic pigs expressing rhEPO in their blood gave rise to higher RBC numbers and HCT contents than control animals. rhEPO was secreted both in the blood and milk of genetically engineered pigs harboring rhEPO gene. Therefore, this study provides a model regarding the production of transgenic pig carrying hEPO transgene for biomedical research.
4,000원
7.
2003.12
구독 인증기관 무료, 개인회원 유료
Park, J. K, Y. K. Lee, P. Y. Lee, S. W. Kim, S. J. Kim, H. G. Lee, J. H. Han, H. K. Chung, C. G. Park
This study was performed to test the cellulose digestibility using the transgenic pigs harboring cellulose degradation gene D (CelD). After delivered offsprings between normal pig and transgenic swine, DNA was isolated from piglets tail for PCR analysis. In first generation, five out of 65 piglets showed CelD positive. Unfortunately, four CelD-positive pigs were died during growing, but one survived pig was used as a transgenic founder to produce F₁ descendents. Among 3 F₁ transgenic pigs produced, one died and the remaining two pigs were used to test the fiber digest efficiency. An assorted feed was composite of 5％ fiber with other ingredients. The feed of 3 kg per day was provided to the pigs including transgenic founders and littermate controls. The manure quantity was measured daily for a month, and all manures were dried for three days to analysis nitrogen, phosphate and fiber concentrations. The fiber digestion efficiencies of the transgenic F₁ pigs showed approximately 10％ higher than those of control pigs. Fiber digestion was not greatly improved in transgenic pigs as it had been expected approximately 30％. Nitrogen concentration of transgenic pig′s manure was slowly decreased compare to the control pigs. Because there were only two transgenic pigs tested, a large number of transgenic pigs may be necessary to obtain more reliable data. Breeding of animals to obtain sufficient transgenic pigs subjected for a further study is on progress. Taken together, this study demonstrated successful production of transgenic pigs with increase of cellulose digestibility in the porcine feed.
4,000원
8.
2003.12
구독 인증기관 무료, 개인회원 유료
Transgenic mice containing GH Receptor (GHR) gene fused to metallothionein promoter were analyzed to evaluate effect of GHR expression on growth in vivo. Three founder mice lines contained copies of GHR transgene and transmitted these genes into F₁ and F₂ progenies. The mRNA expression of transgene was identified using RT-PCR with GHR genes in tissues. To analyze the effects of transgenes on growth performance, body weights of pups were measured at 4, 10 and 14 weeks after birth. The body weight of transgenic mice was higher compared with that of non-transgenic control mice regardless of sex (P<0.05). Body weights between transgenic and non-transgenic mice were increased with aging. Overall, GHR transgenic mice tended to grow about 10 to 15 ％ faster than non-transgenic mice without any pathological defects.
4,000원
9.
2003.12
구독 인증기관 무료, 개인회원 유료
The purpose of this study was to investigate the development of bovine nuclear transfer (NT) embryos cultured in serum-free conditions. Bovine NT embryos cultured in various culture conditions were compared blastocyst development, total cell number and apoptosis using TUNEL assay. In experiment 1, blastocyst rates of NT embryos were significantly higher (P<0.01) in FBS (22.0％) and BSA (26.6％) groups than in PVA (6.3％) group. Total cell number was significantly higher in FBS (78.4±19.4) and BSA (90.9±29.1) groups than in PVA group (46.0±0.0). Apoptotic cell number was significantly fewer in FBS (3.1±1.4) and BSA (1.7±1.4) groups than in PVA group (7.0±20.0) However, all of results were not different between the FBS and BSA group. In experiment 2, blastocyst rates of NT embryos were significantly higher (P<0.05) in fatty acid free-BSA (FAF-BSA) group (26.8％) than in fraction V-BSA group (11.2％). Total cell number were somewhat higher in FAF-BSA group (89.8±30.7) than in fraction V-BSA group (88.1±19.3). Apoptotic cell number were somewhat fewer in FAF-BSA (1.7±1.5) group than in fraction V-BSA group (4.2±2.9). These findings suggest that serum free condition were effective for the in vitro development of bovine NT embryos. Therefore, we concluded that fatty acid free-BSA has beneficial effect in development bovine NT embryos and can be use as a serum substitute.
4,000원
10.
2003.12
구독 인증기관 무료, 개인회원 유료
Although nuclear transfer (NT) techniques are used to clone animals, its efficiency is very low. Moreover, nuclear transfer has resulted in offspring with severe developmental problems, probably due to incomplete nuclear reprogramming. Nuclear reprogramming is characterized by functional modification of the transferred nucleus to allow it to direct normal embryo development with the potential to grow to term. Although the nature of the reprogramming factor(s) in mammals is not clear, various nuclear as well as cytoplasmic components are involved in the processes. In this article we review recent data on factors involved in the nuclear reprogramming of cloned embryos.
4,000원
