간행물
Reproductive & developmental biology
- 발행기관 한국동물번식학회
- 자료유형 학술지
- 간기 계간
- ISSN 1738-2432 (Print)2288-0151 (Online)
- 수록기간 1977 ~ 2018
- 주제분류 농수해양 > 축산학 농수해양 분류의 다른 간행물
- 십진분류KDC 527DDC 636
권호리스트/논문검색
Volume 33 No 3 (2009년 9월) 12건
1.
2009.09
구독 인증기관 무료, 개인회원 유료
The two dimensional quantitative structure-activity relationships (2D-QSARs) models concerning the binding affinity constants (p[Od.]50) between 2-cyclohexyltetrahydropyrane and 2-cyclohexyltetrahydrofurane analogues as substrates, and bovine odorant binding protein (bOBP) as receptor were derived by multiple regression analyses method and discussed. The statistical quality of the optimized 2D-QSAR model (5) was good (r=0.907). From the model, the binding affinity constants (p[Od.]50) were dependent upon the optimal value ((TL)opt.=2.737) of total lipole (TL) of substrate molecules. Based on these findings, the high active compounds predicted by optimized 2D-QSAR model (5) were 2-(dimethylcyclohexyl)tetrahydropyrane molecule and their isomer molecules. The binding affinity constants regarding bOBP of the tetrahydrofuryl-2-yl family compounds were dependent upon the hydrophobicity (logP) of whole substrate molecules. In any case of porcine odorant-binding proteins (pOBP), the constants were dependent upon the hydrophobicity (πx=logPx－logPH) of substituents (R) in substrate molecules. Also, from the optimal values of hydrophobic constant, the hydrophobicity for bOBP influenced ca. twice time bigger (bOBP>pOBP) than that for pOBP.
4,000원
2.
2009.09
구독 인증기관 무료, 개인회원 유료
The aim of this study is to investigate the effect of porcine epididymal fluid (pEF) on in vitro-maturation and subsequent fertilization of porcine follicular oocytes. Porcine cumulus-oocytes complexes retrieved from antral follicles were cultured in tissue culture medium (TCM)-199 supplemented with pEF of different concentrations. At 48 h after culture, development of oocytes to germinal vesicle (GV) breakdown, metaphase I, anaphase-telophase I, and metaphase II were examined. Significant (p<0.05) increase in the proportion of oocytes developed to MII stage was observed in oocytes cultured in pEF-containing TCM-199 than in oocytes cultured in pEF-free TCM-199 (46.2% vs 16.7%), which was a dose-dependent manner. Subsequently, the proportion of monospermic fertilization were significantly (p<0.05) increased in oocytes cultured in the TCM supplemented with pEF than those cultured in pEF-free TCM-199 (51.0% vs 24.1%). In the second series of experiment, the percentage of MII oocytes was significantly (p<0.05) increased after exposure of oocytes to pEF during the first 22 h period of culture than after exposure of oocytes to pEF during the next 24 h of culture, while no significant difference in the percentage of monospermy was observed. The results of this study demonstrate that pEF contains at least enhancing component(s) for nuclear maturation.
4,000원
3.
2009.09
구독 인증기관 무료, 개인회원 유료
Pluripotent embryonic stem (ES) cells isolated from inner cell mass (ICM) of blastocyst-stage embryos are capable of differentiating into various cell lineages and demonstrate germ-line transmission in experimentally produced chimeras. These cells have a great potential as tools for transgenic animal production, screening of newly-developed drugs, and cell therapy. Miniature pigs, selectively bred pigs for small size, offer several advantages over large breed pigs in biomedical research including human disease model and xenotransplantation. In the present study, factors affecting primary culture of somatic cell nuclear transfer blastocysts from miniature pigs for isolation of ES cells were investigated. Formation of primary colonies occurred only on STO cells in human ES medium. In contrast, no ICM outgrowth was observed on mouse embryonic fibroblasts (MEF) in porcine ES medium. Plating intact blastocysts and isolated ICM resulted in comparable attachment on feeder layer and primary colony formation. After subculture of ES-like colonies, two putative ES cell lines were isolated. Colonies of putative ES cells morphologically resembled murine ES cells. These cells were maintained in culture up to three passages, but lost by spontaneous differentiation. The present study demonstrates factors involved in the early stage of nuclear transfer ES cell isolation in miniature pigs. However, long-term maintenance and characterization of nuclear transfer ES cells in miniature pigs are remained to be done in further studies.
4,000원
4.
2009.09
구독 인증기관 무료, 개인회원 유료
This study tried to develop the system (device) that automatically notify a manager of condition just before and after farrowing to extend ubiquitous-based technology and to increase efficiency of delivery care and productivity by reducing human labor and time on standby when farrowing management is done in the difficult and hard working environment of farrowing such as night or holidays in field sand especially in pig industry. In this test, selected 10 gilts were executed timed artificial insemination and were set up each temperature sensor and load sensor to them 3 days before the estimated farrowing day and were observed the farrowing situation. This study was embodied the NESPOT-based (KT Corporation) monitoring system, the system to transmit data in real time by utilization of wireless LAN and the sensor module to apply the ubiquitous environment to them. And this study was observed the situation to automatically notify situations of 10 gilts that first bore just before and after farrowing. The result obtained the farrowing situations of them in real time by setup of the NESPOT-based monitoring system to check farrowing situation directly is as follow. The average time of the automatic notice about situation just before farrowing by the temperature sensor was 27.5 minutes before the beginning of farrowing (the expulsion time of a piglet). 6 of 8 pregnant gilts that first bore automatically were notified situations just before farrowing and the temperature sensors inserted into 2 ones before farrowing were omitted. (The automatic notice rate 75%) The average time of the automatic notice of situation just after farrowing by the load sensor was taken 46.5 minutes after the beginning of farrowing (the expulsion time of a first piglet). The average gestation period of 8 ones that first bore and were tested by the automatic notice of farrowing situation was 115.6 days. This result found that the automatic farrowing notice system by the temperature sensor is more efficient than the load sensor as the automatic farrowing alarm device and sanitary treatment and improvement of the omission rate were required.
4,000원
5.
2009.09
구독 인증기관 무료, 개인회원 유료
Lysophosphatidic acid (LPA), a simple phospholipid-derived mediator implicated in diverse biological actions, acts through the specific G-protein coupled receptors, LPA receptor (LPAR) 1～5. Our previous study showed that LPAR3 is expressed in the uterine endometrium in a cell type- and stage-specific manner and LPA via LPAR3 increases PTGS2 expression in the uterine endometrium during the period of implantation. Although LPAR3 is considered to be predominant LPA receptor in the uterine endometrium, other LPA receptors might play a role to mediate LPA functions in the uterine endometrium during pregnancy. Among LPARs, we investigated expression of LPAR1 during the estrous cycle and pregnancy in this study. Uterine endometrial tissue samples were collected from day (D) 12 and D15 of the estrous cycle and from D12, D15, D30, D60, D90 and D114 of pregnancy. Northern blot analysis determined that LPAR1 mRNA was constitutively expressed in the uterine endometrial tissues during the estrous cycle and pregnancy of all stages. Analysis by immunoblotting revealed that LPAR1 proteins were present in the porcine uterine endometrium during the estrous cycle and pregnancy. Immunohistochemical experiments demonstrated that LPAR1 protein was localized to endometrial epithelium and stromal cell, specifically to nuclei of these cell types. Results in this study show that LPAR1 is constitutively expressed in the uterine endometrium during the estrous cycle and pregnancy. These results suggest that LPA via LPAR1 may play a role in the uterine endometrial function throughout pregnancy in pigs.
4,000원
6.
2009.09
구독 인증기관 무료, 개인회원 유료
Sung-Jai Park, Kwang-Soo Baek, Hyeon-Shup Kim, Byeong-Soon Jeon, Tai-Young Her, Hyun-Joo Lim, Seok-Jin Kang, Hyun-June Lee, Kwang-Sook Ki, Woon-Mo Jo
The purpose of this study was to assess for re-breeding concentrate period in postpartum in milking cows. The 48 cows aged 3.5～5.5 years and of 400～600 kg body weight were examined every 3rd day from 15 to 36 day postpartum. Blood samples for progesterone and estradiol 17 β hormone analyses were withdrawn from the coccygeal vein every third day until the end of the experiment. The ovarian follicular numbers were verified and measured using a multi frequency probe. The least squares means are presented for each day by GLM of SAS. The results showed that ovary lengths (right ovary; 1.64±0.62 cm, left ovary; 1.44±0.46 cm) were similar in right and left ovary activity level during estrous cycle of postpartum cows. We were judged completed uterus on day at 2.31±0.17 cm level of cervix diameter. And we were monitoring started at 6.44±2.03 cm from day 15 after postpartum. The results showed that mean plasma concentration of progesterone (3.28 ng/ml) in large follicle gradually increased days 30 in postpartum. And, monitoring of estradiol 17β (22.18 pg/ml) hormone during postpartum period would be useful to predict the ovarian and uterus activity for re-breeding in postpartum milking cows. From these results, we conclude that cervix diameter (mean: 2.31 cm) was very important for reproductive organ recovery standard level of postpartum milking cows, hormone secretion level (P4: 3.28 ng/ml, E2: 22.18 pg/ml) and body condition score (2.5～2.75) level about 30 days in postpartum period.
4,000원
7.
2009.09
구독 인증기관 무료, 개인회원 유료
Joo-Hyun Shim1, Mi-Rung Park, Byoung-Chul Yang, Yeoung-Gyu Ko, Keon Bong Oh, Jeong-Woong Lee, Jae-Seok Woo, Eung-Woo Park, Soo-Bong Park, Seongsoo Hwang
This study was performed to comprehend the developmental characteristics of cloned embryos knocked out (KO) of α-1,3-galactosyltransferase (GalT) gene. Immature oocytes were collected and cultured for 40 hrs (1-step) or 20hrs (with hormone) + 20hrs (without hormone) (2-step). The embryos transferred with miniature pig ear fibroblast cell were used as control. The reconstructed embryos were cultured in PZM-3 with 5% CO2 in air at 38.5℃ for 6 days. To determine the quality of the blstocysts, TUNEL and quantitative realtime RT-PCR were performed. The embryos were transferred to a surrogate (Landrace) at an earlier stage of the estrus cycle. The maturation rate was significantly higher in 2-step method than that of 1-step (p<0.05). The blastocyst development of GalT KO embryos was significantly lower than that of normal cloned embryos (p<0.05). The total and apoptotic cell number of GalT KO blastocysts was not different statistically from control. The relative abundance of Bax-α/Bcl-xl ratio was significantly higher in both cloned blastocysts than that of in vivo blastocysts (p<0.05). Taken together, it can be postulated that the lower developmental potential and higher expression of apoptosis related genes in GalT KO SCNT embryos might be a cause of a low efficiency of GalT KO cloned miniature pig production.
4,000원
8.
2009.09
구독 인증기관 무료, 개인회원 유료
Joo-Heon Kim, Cheol-Soo Shim, Jinyoung Won, Youngji Park, Sookyoung Park, Jae Seon Kang, Yonggeun Hong
Many biological systems are regulated by an intricate set of feedback loops that oscillate with a circadian rhythm of roughly 24 h. This circadian clock mediates an increase in body temperature, heart rate, blood pressure, and cortisol secretion early in the day. Recent studies have shown changes in the amplitude of the circadian clock in the hearts and livers of streptozotocin (STZ)-treated rats. It is therefore important to examine the relationships between circadian clock genes and growth factors and their effects on diabetic phenomena in animal models as well as in human patients. In this study, we sought to determine whether diurnal variation in organ development and the regulation of metabolism, including growth and development during the juvenile period in rats, exists as a mechanism for anticipating and responding to the environment. Also, we examined the relationship between changes in growth factor expression in the liver and clock-controlled protein synthesis and turnover, which are important in cellular growth. Specifically, we assessed the expression patterns of several clock genes, including Per1, Per2, Clock, Bmal1, Cry1 and Cry2 and growth factors such as insulin-like growth factor (IGF)-1 and -2 and transforming growth factor (TGF)-β1 in rats with STZ-induced diabetes. Growth factor and clock gene expression in the liver at 1 week post-induction was clearly increased compared to the level in control rats. In contrast, the expression patterns of the genes were similar to those observed after 5 weeks in the STZ-treated rats. The increase in gene expression is likely a compensatory change in response to the obstruction of insulin function during the initial phase of induction. However, as the period of induction was extended, the expression of the compensatory genes decreased to the control level. This is likely the result of decreased insulin secretion due to the destruction of beta cells in the pancreas by STZ.
4,000원
9.
2009.09
구독 인증기관 무료, 개인회원 유료
Spermatogonial stem cells(SSCs) only are responsible for the generation of progeny and for the transmission of genetic information to the next generation in male. Other in vitro studies have cultured SSCs for proliferation, differentiation, and genetic modification in mouse and rat. Currently, information regarding in vitro culture of porcine Germline Stem Cell(GSC) such as gonocyte or SSC is limited and is in need of further studies. Therefore, in this study, we report development of a successful culture system for gonocytes of neonatal porcine testes. Testis cells were extracted from 10～14-day-old pigs. These cells were harvested using enzymatic digestion, and the harvested cells were purified with combination of percoll, laminin, and gelatin selection techniques. The most effective culture system of porcine gonocytes was established through trial experiments which made a comparison between different feeder cells, medium, serum concentrations, temperatures, and O2 tensions. Taken together, the optimal condition was established using C166 or Mouse Embryonic Fibroblast(MEF) feeder cell, Rat Serum Free Medium(RSFM), 0% serum concentration, 37℃ temperature, and O2 20% tension. Although we discovered the optimal culture condition for proliferation of porcine gonocytes, the gonocyte colonies ceased to expand after one month. These results suggest inadequate acquirement of ingredients essential for long term culture of porcine GSCs. Consequently, further study should be conducted to establish a successful long-term culture system for porcine GSCs by introducing various growth factors or nutrients.
4,000원
10.
2009.09
구독 인증기관 무료, 개인회원 유료
This study was carried out between November 2007 and October 2008 in the experimental farm of Chunnam National University to investigate the effect of feeding of whole crop barley silage on the reproductive performance of Hanwoo heifers and cows. Two diets, rice straw or whole crop barley silage separately from concentrate were fed 11 Hanwoo heifers and 26 Hanwoo cows. In control group (=CON), heifers (n=6) and cows (n=13) were fed 7 kg (/head) rice straw and 4 kg (/head) commercial diet. In whole crop barley silage group (=WBG), heifers (n=5) and cows (n=13) were fed 8 kg (/head) whole crop barley silage and 1 kg (/head) commercial diet. 1. Conception rates for first service in CON or WBG heifers were 66.7% (4/6) and 60.0% (3/5), respectively, and the services per conception cows were 1.5±0.2 for CON and 1.4±0.2 for WBG group. 2. Days to post-partum insemination were 106.6±26.3 days for CON and 85.6±12.6 days for WBG group, and days to post-partum conception in CON or WBG were 128.4±27.1 and 96.8±16.8 days, respectively. 3. Post-partum conception rates for first service in CON or WBG were 76.9% (10/13) and 84.6% (11/13), respectively, and caving interval was 418.1±50.7 days for CON and 392.8±20.7 days for WBG group.
3,000원
11.
2009.09
구독 인증기관 무료, 개인회원 유료
Changing Proteins in Granulosa Cells during Follicular Development in Pig In-Soon Chae1, Dong-Min Jang3, Hee-Tae Cheong2, Boo-Keun Yang1 and Choon-Keun Park1,† 1College of Animal Life Sciences, 2School of Veterinary Medicine, Kangwon National University, Chuncheon 200-701, Korea 3CHA Stem Cell Institute, CHA Hospital, Seoul 463-712, Korea ABSTRACT This study analyzed change of proteins in granulosa cells during the porcine follicuar development by proteomics techniques. Granulosa cells of the follicles, of which the diameter is 2～4 mm and 6～10 mm, were collected from ovary of slaughtered pig that each follicle of diameter 1～4 mm and 6～10 mm. We extracted glanulosa cell proteins by M-PER Mammalian Protein Extraction Reagent. Proteins were refined by clean-up kit and quantified by Bradford method until total protein was 200 μl. Immobilized pH gradient(IPG) strip used 18 cm, 3～10 NL. SDS-PAGE used 10% acrylamide gel. After silver staining, Melanie 7 and naked eye test were used for spot analyzation. Increasing proteins in glanulosa cell of 6～10 mm follicle were 7 spots. This spots were analyzed by MALDI-TOF MS and searched on NCBInr. In results, 7 spots were similar to zinc/ling finger protein 3 precursor (RING finger protein 203), angiomotin, heat shock 60 kDa protein 1 (chaperonin) isoform 1 (HSP60), similar to transducin-like enhancer protein 1 (TLE 1), SH3 and PX domains 2A (SH3PXD2A). Those proteins were related with transfer between cells. Increase of proteins has an effect on follicular development.
4,000원
12.
2009.09
구독 인증기관·개인회원 무료
