The acute and subacute toxicities of copper sulfate were evaluated in zebrafish (Brachydanio rerio). Dipping of fishes for acute toxicity was performed for a period of 24 h, and TLm24h value (median tolerance limit = LC50) was 1.36 ppm. Clubbing of gill filaments due to severe epithelial hyperplasia of gill lamella were oberved. And epithelial edema, fusion and necrosis of renal tubules were presented. The most significant change was mild epithelial hyperplasia of gill lamella in subacute toxicity test which fishes were exposed to 0.15 ppm of copper sulfate for 1 week.

• 이제엽(충북대학교 수의과대학 수의학과 수생동물질병학연구실) | Je-Yeop Lee
• 허강준(충북대학교 수의과대학 수의학과 수생동물질병학연구실) | Gang-Joon Heo Corresponding author