The objective of this study was to determine the effect of administration of Prostaglandin F2α (PGF2α) on semen collection training and semen characteristics in sexually inexperienced boars. Boars were moved individually to a semen collection pen and were trained to mount dummy sow. During the first and second semen collection secessions, 4 out of 17 boars and 4 out of remaining 13 boars allowed collection of semen. The 9 boars that failed semen collection from first 2 attempts received immediately 15 mg of PGF2α i.m. (intramuscular injection) upon entering the collection pen for semen collection resulted in successful semen collection from all 9 boars. Total numbers of spermatozoa were higher in PGF2α treated boars but there was no significant difference in % motility kinematics characteristics between control and PGF2α treated groups during 72 hr period. Overall, administration of PGF2α in sexually inexperienced boars increased the sex drive and facilitated the mounting activity to the dummy sow for semen collection.