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        검색결과 72

        61.
        2016.11 KCI 등재 서비스 종료(열람 제한)
        감마나이프 방사선수술은 일회에 고방사선량을 조사하는 치료 전략에 기초하기 때문에 렉셀감마플랜의 결과물에 대한 독립적 인 검증은 환자의 안전성을 보장하고 치료 오류의 위험을 최소화하는데 중요한 절차 중 하나이다. 기존에 개발된 여러 검증 방법들의 구현을 통해 통계적으로 검토하고 치료에 시도했다. 이 연구의 목적은 감마나이프 치료에 대해 제안된 여러 검증방법의 정확도를 적용하고 평가하는 것이었다. 본 연구에서 감마나이프방사선 수술에 의해 치료된 두개 내 병변을 가진 10명의 환자가 포함되었다. 우리는 최대 선량, 임의의 점에서의 선량, 등선량중심점에서의 치료시간에 관하여 제안된 알고리즘과 렉셀감마플랜으로 획득한 데이터를 비교 하 였다. 모든 데이터는 두 개의 상이한 측정 기법을 비교하기 위해 사용되는 통계적 방법인 대응표본 t 검정에 의해 분석되었다. 10가지 사례에서 최대 선량의 통계적 유의성은 제안된 검증방법과 렉셀감마플랜 사이에 관찰되지 않았다. 평균 최대 선량의 차이는 –0.53 Gy에서 3.71 Gy범위내였다. 제안된 검증방법과 렉셀감마플랜에 의해 계산된 임의의 점에서의 선량 또한 통계적으로 유의하지 않았다. 그러나 우리는 등선량중심점에서의 치료시간에 대한 조직최대비율 알고리즘과 렉셀감마플랜사이에 p= 0.021인 통계적인 차이가 발견되었다. 통계적 분석에 의하면 제안된 검증방법은 감마나이프 방사선 수술의 최상의 치료계획을 위해 최대 선량과 임의의 점에서의 선량을 고려할 때 렉셀감마플랜과 상당히 일치한다고 볼 수 있다. 제안된 검증방법들은 과다선량 조사 가능성을 최소화하기 위한 통상적인 정도관리절차의 일부분으로 통합될 수 있다고 여겨진다.
        62.
        2014.07 서비스 종료(열람 제한)
        Chinese cabbage is one of most important vegetable crop in Eastern Asian countries including Korea. Because Chinese cabbage is a leafy vegetable, genetic research with respect to the leaf morphology is important. In this research, we have used two inbred lines of Chinese cabbages (Kenshin and RCBr) and generated recombinant lines having various leaf morphology. In F2 population of Kenshin X RCBr, leaf shape showed very dramatic variations with normal distribution in terms of leaf size, petiole length, leaf margin and etc. Microarray with a 135K DNA chip (version 3) integrated 2 sets of total Chinese cabbage genes. Biological process of candidate genes was classified into transcription factor, genes encoding kinase activity protein, protein folding related genes, oxidation-reduction process genes. Putative leaf-morphology-related genes were 142 that are involed in phytohormone pathway genes, cell proliferation & cell elongation related genes and genes controlling leaf morphogenesis etc. These genes are further classified to phytohormone signaling-associated genes (SAUR44, PIN2, CPK6, RDUF2), leaf development regulating genes (DWF4, CUC2, TCP15, BLH4, NGA4), and cell division and cell growth related genes (ILP1, TCTP, EMB1027).
        63.
        2014.07 서비스 종료(열람 제한)
        To select genes associated with the high-temperature tolerance from Brassica, two transcriptomic analyses have been used: microarray and RNA Seq. Using two contrasting inbred lines of B. rapa, Chiifu and Kenshin, version 3 microarray (135 K microarray) was conducted to RNA samples extracted from series of 45℃-treated leaves and 29 genes were selected for genomic DNA cloning of cabbage. Of 29 genes, 8 genes contain 40 SNPs, 11 SSRs and 23 In-Del markers that distinguish high-temperature tolerant and susceptible cabbages, BN1 and BN2. These 8 genes include a unknown gene, AP2, SMP, FBD, SKP2B, IAA16, HSP21 and OLI2-2. We also selected 16 cabbage genes from RNA Seq analysis using two inbred lines, BN1 and BN2: 5 genes for BN1-high expression, 5 genes for BN1-specific expression, 5 genes for BN2-specific expression, and BoCaMB. Using RNA sequences, genomic DNAs corresponding to 16 genes have been clones and analyzed to find out molecular markers. Markers were further transformed into PCR-based marker and confirmed with additional cabbage genetic lines. We are currently transforming PCR-makers into SNP markers. To examine function of high-temperature tolerant genes, we also transformed 5 genes into Arabidopsis plants. We will describe detailed methods and results in a poster. [This work was supported by a grant from the Next-Generation BioGreen 21 Program (the Next-Generation Genomics Center No. PJ009085), Rural Development Administration, Republic of Korea]
        64.
        2014.07 서비스 종료(열람 제한)
        Shaggy-like kinases (SKs), also known as Glycogen synthase kinase 3 (GSK3) proteins, play many important roles in cellular signaling in animals, fungi and amoebae. In particular, SKs participate in key developmental signaling pathways and also regulate the cytoskeleton. SKs -encoding genes are also present in all land plants and in algae, raising questions about possible ancestral functions in eukaryotes. Unlike in animals and Dictyostelium, land plant SKs are encoded by relatively large multi-gene families whose members share high sequence similarity. Along with the studied 10 ASKs (Arabidopsis shaggy-like kinases) indicate that plant SK proteins are actively implicated in hormonal signalling networks during development as well as in biotic and abiotic stress responses. In this study, 18 BrSKs are identified from Chinese cabbage, and they are classified into four groups according to the classification of Arabidopsis. The characterization, classification, gene structure and phylogenetic construction of BrSK proteins are performed. Distribution mapping shows that BrSKs are absented in A02 and A10 chromosome. 8 orthologous gene pairs are shared by Chinese cabbage and Arabidopsis. The expression patterns of BrSK genes exhibit differences in five tissues based on RNA-seq data in public data base. Specially, BrSKβ-1 and BrSKβ-2 show floral buds specifically expressed, which indicate that BrSKβ may play a key role during flower or pollen development. We deomonatrated that suppresion of Arabdiopsis orthology of BrSKβ impaired the late pollen in Arabidopsis plants. Taken together, our analyses provided insights into the characterization of the BrSK genes in Chinese cabbage, providing foundation of further functional studies of those genes. [This work was supported by a grant from the Next-Generation BioGreen 21 Program (the Next-Generation Genomics Center No. PJ008118), Rural Development Administration, Republic of Korea]
        65.
        2012.07 서비스 종료(열람 제한)
        Cold stress, which includes chilling (<20℃) and/or freezing (<0℃) temperatures, adversely affects the growth and development of plants and significantly constraints the spatial distribution of plants and agricultural productivity. Cold signal in plants is transmitted to activate C-repeat/drought-responsive elements-binding factor (CBF)-dependent and independent transcriptional pathway. In Arabidopsis, cold-regulated genes have been estimated to constitute ~4% to 20% of the genome. Chinese cabbage (Brassica rapa ssp. pekinensis), like Arabidopsis, is a member of the Cruciferae family. With expectation that Chinese cabbage has similar cold-responding process as Arabidopsis, transcriptome profiles were examined in two comparable DH lines, Chiifu and Kenshin, using 24K microarray and expression of some of genes was analyzed by RT-PCR. Hundreds genes showed over two fold change upon freezing treatment, but only four genes specific for each line. Most of examined CBF-dependent and -independent pathway related genes have a similar expression patterns between Chiifu and Kenshin, except BrICE1 (inducer of CBF expression 1), MYB15, BrRAP2.1 and BrRAP2.6 (ethylene-responsive transcription factor 2.6). The BrICEL (inducer of CBF expression 1 like) showed specifically expression in Kenshin. The expression levels of vernalization related genes (BrRTV1, BrVRN5, BrVIN3L, VIP3 and VIP5) showed no difference between Chiifu and Kenshin and did not response to freezing treatment in our experiment. We will discuss more detailed expression data on poster.
        66.
        2012.07 서비스 종료(열람 제한)
        Abiotic stresses such as extreme temperatures frequently limit the plant growth and productivity of major crop species. Two Chinese cabbage DH lines that have different geographic origins, in that Chiifu is from temperate regions, while Kenshin is from subtropical and tropical regions have been expected to show the specific response to high or low temperature. To find the temperature response genes between Chiifu and Kenshin, we analyzed transcriptomic profiling from light-chilling (6h at 4°C) and high temperature (6h at 38°C) treated plants using the KBGP-24K chip. Distribution of genes classified by PI (probe intensity) values showed remarkable difference between Chiifu and Kenshin. The number of genes up- and down-regulated gens by both temperatures were 135 and 79 genes, respectively. These genes may be temperature stress-related genes. Genes involved in the response to stress were changed by light-chilling stress. Chiifu specifically up-regulated genes upon light chilling-stress belong to cold acclimation proteins, calcium binding proteins, cell wall biogenesis proteins and lipoxygenase. On the other hand, Kenshin specifically up-regulated genes by heat-shock treatment include heat-shock proteins, phosphatases, protein folding and phosphorylation-associated ones. Further study on these specific genes function may provide insight to adaptation of Chinese cabbage and clue to develop molecular markers.
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