This study examined pregnancy and fetal loss rates according to different estrus synchronization protocols and injection of gonadotropin releasing hormone (GnRH) after transfer of Korean Native Cattle embryos to Holstein recipients. In Experiment 1, recipients received no treatment (Control, n = 119); two injections of prostaglandin ( ) 11 days apart (PGF group, n = 120); GnRH (day 0)- (day 7)-GnRH (day 9) (Ovsynch group, n = 120); and CIDR (day 0)- and CIDR removal (day 7)-GnRH (day 9) (CIDR group, n = 110). In Experiment 2, the control group was received no treatment of GnRH. The treatment groups were received GnRH at embryo transfer (ET) (day 0), 7 days later, 14 days later, ET and 7 days later, 7 and 14 days later, or ET, 7 and 14 days later. Recipients were assigned to treatment randomly and received two in vitro produced blastocysts. Pregnancy was diagnosed at day 60 by palpation per rectum. Fetal loss to term was determined by palpation every 90 days thereafter. In Experiment 1, the pregnancy rate in the CIDR group (59.1%) were higher than in the Control group (42.0%) (p<0.01); fetal loss rates were similar for all groups (12.0 to 18.5%). In Experiment 2, the pregnancy rate in Day 0+7+14 group was higher (60.2%) than the control (40.2%) (p<0.01) and resulted in a lower fetal loss (p<0.05) than the control (4.6 vs. 11.4%). There were no significant difference between other treatment and the control (p>0.05). These results show that pregnancy rates of bovine embryos can be enhanced by CIDR insertion or GnRH treatment. Additionally, fetal loss may be reduced with GnRH treatment after ET.

This study was carried out to investigate the effects of the supplementation of glutamine, glucosamine and glutathione on the porcine oocytes on IVM rates. Cocs were incubated in NCSU-23 supplemented with at glucosamine, glutamine and glutathione for 48 hrs. Oocytes were transferred to 50 ul drops of maturation medium covered with mineral oil and cultured in a incubator (, 5% , 95% air). The IVM rates of oocytes cultured in NCSU-23 supplemented with 0.5, 1.0, 2.0 and 4.0 mM glutamine for 48 hrs were , , and , respectively. The IVM rates of oocytes cultured in NCSU-23 supplement with 2.0, 5.0, 7.0, 10.0 mM glucosamine for 48 hrs were , , and , respectively. The IVM rates of oocytes cultured in NCSU-23 supplemented with glucosamine were no significantly increased compare to the control (). The IVM rate of oocytes cultured in NCSU-23 supplemented with 3.0, 5.0, 7.0, 10.0 mM glutathione for 48 hrs were , , , , respectively. The IVM rate of oocytes cultured in NCSU-23 supplemented with glutamine and glutathione were significantly increased co~pared to those control (). Glucosamine did not affect the IVM rates of oocytes. IVM rates of oocytes cultured in NCSU-23 medium for 48 hrs were significantly increased compared to the cultured for 40 hrs.

Since the birth of Dolly using fully differentiated somatic cells as a nuclear donor, viable clones were generated successfully in many mammalian species. These achievements in animal cloning demonstrate developmental potential of terminally differentiated somatic cells. At the same time, the somatic cell nuclear transfer (SCNT) technique provides the opportunities to study basic and applied biosciences. However, the efficiency generating viable offsprings by SCNT remains extremely low. There are several explanations why cloned embryos cannot fully develop into viable animals and what factors affect developmental potency of reconstructed embryos by the SCNT technique. The most critical and persuasive explanation for inefficiency in SCNT cloning is incomplete genomic reprogramming, such as DNA methylation and histone modification. Numerous studies on genomic reprogramming demonstrated that incorrect DNA methylation and aberrant epigenetic reprogramming are considerably correlated with abnormal development of SCNT cloned embryos even though its mechanism is not fully understood. The SCNT technique is useful in cloning farm animals because pluripotent stem cells are not established in farm animal species. Therapeutic cloning combined with genetic manipulation will help to control various human diseases. Also, the SCNT technique provides a chance to overcome excessive demand for the organs by production of transgenic animals as xenotransplantation resources. Here, we describe the factors affecting the efficiency of generating cloned farm animals by the SCNT technique and discuss future directions of animal cloning by SCNT to improve the cloning efficiency.

남해안의 경남 진도에 분포하는 전기 백악기 진주층의 호성 실트암에서 약 450여 개의 생흔 화석 Lockeia가 발견되었다. 이 생흔 화석들은 상당히 길쭉한 씨앗 모양이며, 대부분 길이가 60-70 mm, 폭이 15-20 mm이다. 그들은 특징적으로 날카로운 세로 고랑이 양쪽 가장자리에서 급하게 경사진 부분과 접하며, 주변부는 융기되어 있고, 세로 고랑의 양쪽 끝부분은 뽀족하다. 이 생흔 화석은 신종인 Lockeia gigantus ichnosp. nov.로 기재한다. Lockeia gigantus와 함께 드물게 발견되는 담수 이매패류인 Plicatounio는 이 휴식 생흔 화석의 가장 유력한 생성자로 판단된다. 이 신종 생흔화석은 현재까지 알려진 Lockeia 중에서 가장 크고 백악기 육성 퇴적층에서 세계적으로 처음 보고된 Lockeia이다. 이 화석은 또한 얕은 호수 환경의 생물 군집에서 살았던 생흔 화석 생성자 (이매패류인 Plicatounio)와 관련된 휴식 생흔화석의 특이한 예이다.

This study was conducted to investigate the efficacy of vitrification procedure for the cryopreservation of porcine oocytes and the utilization of vitrified oocytes as recipient cytoplasts for somatic cell nuclear transfer (NT), and observed that porcine oocytes are evaluated by pronuclear formation, and parthenogenetic development. Single fetal donor cells were deposited into the perivitelline space of vitrified enucleation oocytes, followed by electrical fusion and activation. NT embryos were cultured in NCSU-23 medium supplemented with 5% FBS, at in 5% and air. 1. When the developmental rates of the oocytes after being culture for hours vitrified with EDS and ETS were 42.0%, 38.0%, respectively. This results were lower than the control group(62.2%). 2. When the developmental rates of the oocytes after being culture for hours vitrified-thawed with sucrose and glucose, 5% PVP, NCSU-23 supplemented with 10% FBS were 33.3%, 25.9%, respectively. This results were lower than the control group(55.6%). 3. The fusion and development to the blastocyst stage between the NT embryos constructed with the vitrified and non-vitrified oocytes were significant differences. Developmental rate of oocytes and NT embryos constructed with the vitrified or non-vitrified oocytes were , respectively.

The aim of present experiment was to examine hatching rate as in vitro indicator of viability of porcine embryos before early stage embryo transfer such as zygotes or 2-cell stage embryos. Cumulus-oocyte complexes (COCs) collected from ovaries were matured in North Carolina State University 23 (NCSU-23) containing 10% porcine follicular fluid (pFF), 10 ng/ml epidermal growth factor (EGF), follicle stimulating hormone (FSH), luteinizing hormone (LH), and 1mg/ml cysteine. After 24 hours, the COCs were transferred to the same medium without hormones. After 65h of maturation, oocytes were exposed to phosphate buffered saline (PBS) with 7% ethanol (v/v) for 7 minutes, and then the oocytes were washed and cultured in tissue culture medium (TCM) 199 containing 5 ug/ml cytochalasin B for 5h at in an atmosphere of 5% and 95% air with high humidity. After cytochalasin B treatment, the presumptive parthenotes were cultured in porcine zygote medium (PZM)-5 and cleavage of the parthenotes was assessed at 72h of activation, Normally cleaved parthenotes were cultured for an additional 8 days to evaluate their ability to develop to blastocyst and hatching stages. The fetal bovine serum (FBS) were added at Day 4 or 5 with concentrations of 2.5, 5 or 10%. The blastocyst rates were ranged within in each treatment. However hatching rate was dramatically decreased in non-addition group. In this experiment, embryo viability in female reproductive tract may be estimated before embryo transfer with in vitro culture adding FBS by hatching ability.

일본 정부는 사용후핵연료의 중간저장시설의 입지로 아우모리현의 무쯔시를 선정했다. 2000년부터 시작된 유치활동이 5 년만에 결실을 맺은 것이다. 한국은 사용후핵연료를 원자력 발전소 내에 저장하고 있는데 2016년이면 저장한계에 이를 것으로 전망되고 있어 일본의 사례연구는 한국에 시사하는 바가 크다. 한국은 경주에 중, 저준위 방사성 폐기물 저장시설을 성공적으로 유치한 경험이 있어 사용후핵연료의 저장시설을 유치하는 학습경험을 축적한 바 있는데 두 나라의 큰 차이점은 한국은 경쟁적인 주민투표를 통하면서 막대한 지역지원금을 지원한다는 점이고 일본은 주민투표를 시행하지 않고 지방자치 정치의 리더쉽이 문제를 해결하는 방식을 택했다는 점이다. 엄청난 지역지원금이 지원되지 않은 점도 일본의 특징이다. 본 연구가 한국의 사용후핵 연료 저장시설을 유치하는 데 도움이 되었으면 하는 바람이다.

Lower Ordovician (Upper Tremadoc) graptolites were found for the first time at the two sections of the Mungok Formation in Danyang area: the Seokgyori and Daejeonri sections. The graptolites from these sections comprise two species of two genera: Adelograptus brevibrachiatus and Psigraptus jacksoni. The graptolite faunas of the sections in the area show simple composition relative to Yeongwol area; they appear to be correlated to: (1) the Adelograptus cf tenellus Zone of Yeongwol area, (2) the Adelograptus cf. tenellus Zone of Yukon, Canada, (3) the Psigraptus Zone of Tasmania, Australia, and (4) the Psigraptus jacksoni Zone of Jilin, China. The age of graptolite beds of the Mungok Formation at Danyang appears to be early Upper Tremadoc of the Lower Ordovician.

Site response analyses were performed based on equivalent linear technique using shear wave velocity profiles of 162 sites collected around the Korean peninsula. The site characteristics, particularly the shear wave velocities and the depth to the bedrock, are compared to those in the western United States. The results show that the site-response coefficients based on the mean shear velocity of the top 30m (V_{S30}) suggested in the current code underestimates the motion in short-period ranges and overestimates the motion in mid-period ranges. The current Korean code based on UBC is required to be modified considering site characteristics in Korea for the reliable estimation of site amplification. From the results of numerical estimations, new regression curves were derived between site coefficients (F_{a}\;and\;F_{v}) and the fundamental site periods, and site coefficients were grouped based on site periods with reasonable standard deviations compared to site classification based on V_{S30}. Finally, new site classification system and modification of design response spectra are recommended considering geotechnical characteristics in Korea.

The concept of Anima Mundi (or World Soul) pervading all of nature is so old that its origins are very obscure. Yeats has kept developing his own philosophy (or system) regarding this Anima Mundi for his art and belief all his life. In this article, I try to treat Yeats's theoretical statements on the Anima Mundi which can be easily found in his essay "Anima Mundi" in Per Amica Silentia Lunae (1918), one of the greatest watershed document in his theory of the Anima Mundi, the poet's ultimate expression of his belief. Like many of ancient western and eastern mysterious religions, Yeats no longer distinguishes between the great memory of Anima Mundi and the minds and memories of its occupants. Also he reaffirms the four elements as integral to the World Soul concept. He assigns elemental attributes to the images glimpsed in visions, creating a fourfold model of perception. This short but important essay constitutes the outstanding feature of Yeats's newly evolved concept, the Anima Mundi is not merely a place, a shadowy subliminal Hades. It is a congeries of souls, of discarnate intelligences whose multifarious activities range from regulating nature's cycles to potential anamnesis.

Early Ordovician graptolites from the Mungok Formation of Yeongwol area, Korea comprise seven species belonging to six genera: Callograptus curvithecalis, Callograptus sinicus, Aspidograptus lotolatzensis, Dendrograptus suni, Dictyonema uniforme, Adelograptus cf. tenellus, and Psigraptus jacksoni. Two graptolite zones with three subzones are recognized in the eight sections of the Mungok Formation. The Adelograptus Zone is correlated with (1) La 1b of the Lancefield Formation of Victoria, Australia, (2) the Adelograptus-Clonograptus Zone of the Yehli Formation of Jilin, China, and (3) the Adelograptus Zone of the Road River Formation of Yukon, Canada. It suggests that the upper part of the Mungok Formation corresponds to early Late Tremadocian of Early Ordovician.

제주도의 후기 플라이스토세 퇴적층에서 깃털 화석을 발견하여 기재하였다. 수심이 얕은 바닷가에서 퇴적된 이 퇴적층에서는 사람 발자국을 포함하여 다양한 새 발자국 화석과 포유류 발자국 화석이 풍부하게 산출되었다. 담회색 이암에 밝은 색의 얇은 막으로 잘 보존된 깃털 화석은 깃 판(vane)이 있는 깃털의 일부분이다. 비록 표본의 크기가 길이 10.3 mm, 폭 9.0 mm로 비교적 작지만, 깃대 양쪽에 두 개의 편평한 깃판(vane), 거의 나란하게 휘어진 깃 가지(barbs)그리고 수많은 전면과 후면의 작은 깃 가지(barbules)들이 잘 보존되어 있다. 이 깃털 화석은 국내에서 최초로 발견되어 기재된 것이며 또한 전 세계의 플라이스토세 퇴적층에서도 처음으로 발견된 것이다.