Pollution in the fresh water system in urban area has the adverse effect on the amphibians population. Restoration activity of amphibian in the urban stream has been growing in Korea as well as western country. For successful restoration water quality of urban stream should be sufficient for survival and normal development of amphibian. To monitor the biological safety of surface water in the Tancheon basin, the capital area of Korea, a 6-day exposure Bombina orientalis embryo developmental toxicity assay was examined. The toxicity of surface water of Tancheon mainstream were lower than those of tributaries of Tancheon. The survival rate of embryos negatively correlated with total dissolved solid, turbidity and electrical conductivity whereas the developmental abnormality and growth retardation of embryos was positively correlated with total dissolved solid, turbidity and electrical conductivity. An amphibian developmental toxicity assay would be helpful for the selection of point for construction of habitat and reintroduction of amphibian in interrupted urban stream.