Fungi have been known as an irritant or causal agent of asthma. Thus, information on their concentration and diversity in indoor air of asthmatic patient’s houses is valuable for the management of these micro-organisms. The present study was performed to investigate fungal concentration and diversity in indoor air of resident spaces in two adult patient’s houses in winter. Air samples were collected in February of 2018 from living rooms and bedrooms of two asthma patients’ houses located in two different places in Incheon city using an impaction method. The average concentrations of airborne fungi did not exceed the 500 CFU/m3 level which is recommended by the Enforcement Managements of the Indoor Air Quality, Ministry of Environment, Korea. A total of four genera and 23 species were identified based on morphological and molecular methods. Aspergillus and Penicillium were two major genera. Aspergillus pseudoglaucus and Penicillium citrinum were commonly found between the two patient’s houses. Among the identified fungi, three species, Aspergillus venenatus, Penicillium jamesonlandense, and Penicillium salami were found to be unrecorded species in Korea. The morphological and molecular characteristics of these three unrecorded species were described. Since these species produce spores well and a lot, they are considered to be a species that needs management. This study provides basic information on the fungi for indoor air quality management in the living spaces of asthma patients in winter in Korea.