This study aimed to obtain basic information on the indoor environmental hygiene of non-disinfected libraries used for paper records preservation in the Nara Repository of National Archives, Korea. Microorganisms were investigated in dust samples collected from bookshelves of five libraries using the swab method. Bacterial concentration ranged from 6 CFU/m2 up to 1,730 CFU/m2 . A total of 11 bacterial species belonging to five genera were identified, with Bacillus being the predominant genus. Some bacterial species forming colonies with pigmentation on TSA media were also present. No bacterial species capable of producing cellulases were found. However, one species that could have harmful effects on human health was discovered. For fungi, concentration ranged between 6 CFU/m2 to 1,660 CFU/m2, and a total of six fungal species belonging to five genera were found. Some fungal species forming pigmented colonies on PDA media were also present. Additionally, three species that could have harmful effects on human health were identified. This study’s data suggests that microbial contamination in the dust is relatively low, but the dust in the bookshelves of non-disinfected libraries at the Nara Repository requires management. This is the first report conducted on microorganisms in the dust of bookshelves at the National Archives in Korea.

ATP luminescence measurements (using Relative Light Units, RLU) has been used to assess the levels of bacterial contamination on the surfaces of various materials. However, not much is known about their suitability in assessing bacterial contamination on paper surfaces. This study was conducted to evaluate the feasibility of using ATP luminometers in measuring levels of bacteria contamination on paper surfaces. The three ATP luminometers studied were Clean-Q, smart PD-30, and 3M™ Clean-Trace™ LM1 manufactured by different companies. There were some differences in RLU results among the three ATP luminometers when they were tested with different concentrations of Micrococcus luteus cell suspension. 106 - 107 cells were required in order to effectively detect Bacillus subtilis, Escherichia coli, and Micrococcus luteus on the surfaces of A4 printing sheets (100 cm2) when using the three ATP luminometers. The sizes and physical properties of surface areas varied slightly among the swabs used for the three ATP luminometers. Concentration-specific measurements (RLU) of M. luteus taken from the surfaces of six kinds of paper (fine print paper, medium print paper, ground paper, newsprint paper, practice paper, tracing paper) were possible using the smart PD-30 and LM1 ATP luminometers. ATP detection values of M. luteus varied among the six types of paper. The highest ATP detection values were found on the surfaces of tracing paper. If the RLU value is recorded at the level of 1000, this could indicate a very high bacterial contamination level of 105 to 106 CFU/4 cm2.

Fungi have been known as an irritant or causal agent of asthma. Thus, information on their concentration and diversity in indoor air of asthmatic patient’s houses is valuable for the management of these micro-organisms. The present study was performed to investigate fungal concentration and diversity in indoor air of resident spaces in two adult patient’s houses in winter. Air samples were collected in February of 2018 from living rooms and bedrooms of two asthma patients’ houses located in two different places in Incheon city using an impaction method. The average concentrations of airborne fungi did not exceed the 500 CFU/m3 level which is recommended by the Enforcement Managements of the Indoor Air Quality, Ministry of Environment, Korea. A total of four genera and 23 species were identified based on morphological and molecular methods. Aspergillus and Penicillium were two major genera. Aspergillus pseudoglaucus and Penicillium citrinum were commonly found between the two patient’s houses. Among the identified fungi, three species, Aspergillus venenatus, Penicillium jamesonlandense, and Penicillium salami were found to be unrecorded species in Korea. The morphological and molecular characteristics of these three unrecorded species were described. Since these species produce spores well and a lot, they are considered to be a species that needs management. This study provides basic information on the fungi for indoor air quality management in the living spaces of asthma patients in winter in Korea.

To obtain information on the indoor air quality of un-disinfected libraries used for paper records preservation, temperature, humidity, and bacterial concentration and species were investigated at five un-disinfected libraries from May to September in 2019 in the National Archives, Seoul (Nara Repository), Korea. Temperature and humidity of all the five un-disinfected libraries were well maintained at 18-22oC and 40-55% in compliance with the National Archives Paper Records Preservation Environment Standard. Bacterial concentration ranged from 2 CFU/m3 up to 280 CFU/m3 which were lower than the Korean indoor air quality standard value. A total of 68 bacterial species belonging to 33 genera were identified from indoor air of the five un-disinfected libraries. Among the 33 genera, Bacillus, Micrococcus, and Staphylococcus were the major genera. Only Micrococcus yunnanensis, Roseomonas mucosa, and Moraxella osloensis were commonly found among the five un-disinfected libraries. Bacterial species producing colonies with color on TSA media were present. Among the 68 species, Bacillus circulans and B. megaterium known to produce cellulases were found. There were also 17 species which have harmful effect on human health. The results of this study indicate that continuous monitoring of air borne bacteria is necessary in the un-disinfected libraries used for paper records preservation.

Mold is one of the harmful biological pollutants in the indoor environment. To investigate how the contamination degree of mold existing in the indoor environment differs according to the characteristics of the house, the concentration of mold in the indoor air was investigated for the houses damaged by leakage or flooding. As a result of a survey of 20 houses located in Gyeonggi-do and Chungcheong-do, the concentration of mold in indoor air surprisingly exceeded more than two times the recommended indoor air quality standard of 500 CFU/m3 in all the houses investigated. The fungal concentration was high in houses with condensation and mold odors. As for the housing type, the pollution degree was higher in detached houses than in row houses. As for the type of water damage, mold contamination was higher in the leaky houses than in the flooded houses. The degree of mold concentration was 1,237 CFU/ m3 in the leaky houses. In terms of housing age, pollution was very high in houses over 20 years old. The occurrence of these measured mold concentrations was correlated at a significant level with respect to the properties of the house. This study shows that in the case of water damaged houses, careful management of mold contamination is required.

2016년과 2017년 서천, 장흥, 부여, 여주에 소재하는 표고 재배사에서 진균성 병원균을 모니터링 하는 과정에서 Acrodontium crateriforme, Naganishia friedmannii, Pestalotiopsis trachicarpicola, Penicillium wollemiicola, Trichoderma thailandicum 등 5 종의 국내 미기록 진균을 재배사내 실내 공기, 버섯 파리, 버섯 배지재료 등에서 분리하였다. 이들 진균은 PDA 배양하여 생장시키면서 형태적 특성 조사하고 26S rDNA, 28S rDNA, β-TUB 유전자, TEF 유전자 등을 PCR 기술로 증폭하고 그 염기서열 분석을 수행하여 동정하였다. 본 논문에서는 이들의 분류학적 위치와 위해성에 대해 보고하고자 한다.

Asthma is a chronic diseases that restricts the air passage ways and which is thought to be caused by several factors such as genetics, obesity, mVOC, air pollution, allergens, and fungi. Domestic information on fungi in the indoor air in the houses of asthma patients in the spring and fall is rarely available. Thus, this study was carried out to investigate fungal concentration and diversity. Air samples were collected from the houses of 6 asthma patient in April and October of 2017 located in Seoul and Gyeonggi Provinces using an impaction method. The average concentrations of suspended fungi in the indoor air at the houses of adult asthma patient in October exceeded the 500 CFU/m3 level recommended by the Enforcement Managements of the Indoor Air Quality Control Act. A total of 70 fungi were isolated and identified. Four genera and 11 species were from the April sampling and 10 genera and 27 species were from the October sampling. Among the identified fungi, five species, Arthrinium marii, Penicillium austrosinicum, Penicillium concavorugulosum, Penicillium exsudans and Penicillium johnkrugii were found to be unrecorded species in Korea. The known properties of all identified fungi and the morphological and molecular characteristics of the five unrecorded species were described.

The use of air cleaners has become popular to quickly remove contaminants present in indoor air such as fine dust and fungi. However, fungal information is rarely available in the filters equipped in the air cleaners. This study was conducted in winter to examine fungal concentration and species in the air cleaner filters of ten residential houses located in Incheon, Seoul, Hwaseong, and Gimpo cities. Fungi were detected in nine out of eleven filters from the ten houses. The level of fungi in the filters ranged from 0 CFU/cm2 to 2,370 CFU/cm2. There were differences in the fungal concentration among the eleven filters. Filters from three houses revealed levels over 1,000 CFU/cm2. The operation time of air cleaners did not seem directly related to the level of fungal concentration. A total of 326 isolates were obtained and classified as six genera belonging to Alternaria, Aspergillus, Cladosporium, Fusarium, Penicillium, and Trichoderma. Among these six genera, 20 species including one Alternaria, one Aspergillus, three Cladosporium spp., two Fusarium spp., eleven Penicillium spp., and two Trichoderma were identified. There were species that cause allergic reaction, pneumoniae, mycosis, and plant disease. Three species were known species of mycotoxin producers. Aspergillus niger, Cladosporium cladosporioides, and Penicillium brevicompactum were the most frequently detected fungi both in the air and in the air cleaner filters. This is the first report of fungal communities present in the air cleaner filters of residential houses in Korea.

Fungus has been regulated as an air contaminant in indoor environments in Korea. To remove the contaminant, air purifiers have been used in houses. However, not much information on the status of fungal contamination in the indoor air of houses is available. The present study was carried out to investigate fungal concentration and species in the indoor air of houses using air purifiers in winter. The results of indoor air sampling of ten houses located in the cities of Incheon, Seoul, Hwaseong, and Gimpo showed that the level of fungi ranged from 9 CFU/ m3 to 782 CFU/m3. Eight out of ten houses showed a level of less than 165 CFU/m3 indicating that the fungal level is generally low. Only one house exceeded the Korean regulatory standard value of 500 CFU/m3. A total of 175 isolates were obtained and classified as three genera belonging to Aspergillus, Cladosporium, and Penicillium. Among these three genera, 15 species including four Aspergillus spp., two Cladosporium spp., and nine Penicillium spp. were identified. Regarding the detection rate of the 15 identified fungal species, A. niger was the most detected species and followed by Cladosporium cladosporioides. Three to six species were present in the individual house that was investigated. Overall, our results showed that the level of fungal concentration and species diversity are low in the indoor air of houses that use air purifiers in winter.

봄철 천식환자 가정집 9곳에서 실내 공기를 포집하여 부유진균의 농도 및 다양성을 조사하였다. 조사된 천식환자 가정집의 평균 부유진균 농도는 거실 143 CFU/m³, 방 142 CFU/m³ 이었으며 외기보다 낮았다. 이는 다중이용시설 실내공기질 기준법의 권고기준 기준치 500 CFU/m³를 초과하지 않았음을 보여준다. 총 59균주를 분리하였으며 5속 33종으로 동정하였다. 이 중 병원균으로서 인체에 위해성을 가지고 있는 진균은 4속 12종이었다. 동정된 종 중 Cladosporium crousii, Penicillium maximae, Aspergillus protuberus는 국내에 보고되지 않은 미기록 진균 종으로 확인하였다. 이 중 A. protuberus는 국외에서 인체에 질병을 유발시키는 균으로 알려진 진균이다. 이상의 결과는 천식환자 가정에서 진균의 관리는 정량적 수준에서뿐만 아니라 유해종을 파악하여 관리하는 정성적인 수준에서도 이루어져야 함을 제시한다. 본 연구는 봄철 천식환자 가정집에 존재하는 실내 공기 중 부유진균의 농도 및 다양성에 대한 최초의 연구이다.

To obtain basic data on bioaerosols in the indoor environments of houses located adjacent to Gwangyang Iron Works, the concentration and diversity of indoor air molds were comparatively investigated in 33 houses in September of 2016 and 2017, respectively. In areas both adjacent to and nonadjacent to Gwangyang Iron Works, house temperature and humidity ranged from 24~28oC and 47~57%, respectively. Airborne mold concentration was higher in the houses located nonadjacent than in the ones adjacent to the iron works. Interestingly, the level of airborne mold concentration exceeded 500 CFU/m3 in all houses nonadjacent to the iron works. A total of 12 mold species including five pathogenic species were indentified from the investigation. Among the five pathogenic species, the causal agents of otomycosis, Alternaria alternata, Aspergillus fumigatus, and A. niger were present. Overall, Cladosporium cladosporioides was the dominant species. This is the first report on the concentration and diversity of airborne mold in houses located adjacent and nonadjacent to Gwangyang bay industrial complex in Korea.

Mold is one of the important bio-aerosols affecting human health in the indoor environment. To manage mold contamination, it is necessary to use an appropriate method for its detection and enumeration. Recently, the impaction method of ISO 16000-18 has been established as one of methods to detect and enumerate molds in air. To investigate the general use of the impaction method for mold detection in domestic indoor environments, the suitability of the method was assessed using different antibiotics, media and air samplers. All of the three antibiotics tested - ampicillin, chloramphenicol and streptomycin - showed inhibitory effects on bacterial colony formation on MEA and DG-18 media, without inhibiting mold growth. Of these three antibiotics, ampicillin was the most effective. There was no statistical difference between MEA and DG-18 media in the measurement of mold concentration. The formation of discriminative colony morphology was more apparent in DG-18 media. No significant difference in the measurement of mold concentration was found between Andersen samplers and MAS- 100NT samplers, which are two major samplers introduced in Korea.

Four fungal species, during indoor air monitoring for fungi that possibly affect the field testing of a newly bred shiitake cultivar in cultivation houses located in Cheongyang, Chungnam Province and Jangheung, Jeonnam Province. Of these species, two are known to be plant pathogens and the other two are saprobes but potent contaminators in the mushroom cultivation environment. This study reports the morphological characteristics and phylogenetic relationships of these four species based on nucleotide sequences of the internal transcribed spacer (ITS) and 18S rDNA region, including their known information.