To understand microorganism effects on wild mushroom fruiting bodies, we investigated the fungi in hyphosphere soil supporting wild mushroom species Cortinarius violaceus, Amanita hemibapha, Laccaria vinacelavellanea, and Amanita verna found in the Gotjawal area of Jeju Island. Fungal species identification based on morphological traits and molecular analysis of ITS, LSU rDNA, and -tubulin gene sequences resulted in isolation and identification of eleven fungal species previously unrecorded in Korea. These newly-recorded species are: Arthrinium kogelbergensis, Kalmusia longisporum, Keithomyces carneum, Neopyrenochaeta cercidis, Penicillium ranomafanaense, Phomatodes nebulosa, Pyrenochaeta nobilis, Tolypocladium album, Talaromyces kendrickii, Talaromyces qii, and Umbelopsis gibberispora, and their morphological characteristics and phylogenetic positions are described.

ATP luminescence measurements (using Relative Light Units, RLU) has been used to assess the levels of bacterial contamination on the surfaces of various materials. However, not much is known about their suitability in assessing bacterial contamination on paper surfaces. This study was conducted to evaluate the feasibility of using ATP luminometers in measuring levels of bacteria contamination on paper surfaces. The three ATP luminometers studied were Clean-Q, smart PD-30, and 3M™ Clean-Trace™ LM1 manufactured by different companies. There were some differences in RLU results among the three ATP luminometers when they were tested with different concentrations of Micrococcus luteus cell suspension. 106 - 107 cells were required in order to effectively detect Bacillus subtilis, Escherichia coli, and Micrococcus luteus on the surfaces of A4 printing sheets (100 cm2) when using the three ATP luminometers. The sizes and physical properties of surface areas varied slightly among the swabs used for the three ATP luminometers. Concentration-specific measurements (RLU) of M. luteus taken from the surfaces of six kinds of paper (fine print paper, medium print paper, ground paper, newsprint paper, practice paper, tracing paper) were possible using the smart PD-30 and LM1 ATP luminometers. ATP detection values of M. luteus varied among the six types of paper. The highest ATP detection values were found on the surfaces of tracing paper. If the RLU value is recorded at the level of 1000, this could indicate a very high bacterial contamination level of 105 to 106 CFU/4 cm2.

Fungi are organisms that must be monitored and controlled in order to preserve valuable paper records. They reduce the quality of paper records by degradation and/or discoloration. As an effort to understand fungal contamination in the National Archives of Korea, the concentration of fungi in the indoor air of the unsterilized record rooms has been reported. However, what species are present in the facilities is not much known. In the present study, we examined the fungi isolated from the National Archives of Korea and found among them that there are fungal species which are newly documented in Korea. They were identified based on morphological properties and nucleotide sequences analysis of the polymerase chain reaction-amplified the internal transcribed spacer region of rDNA, calmodulin gene, and beta-tubulin gene. We report Cladosporium parahalotolerans, Cladosporium subuliforme, Ochroconis mirabilis, Penicillium angulare, and Penicillium fundyense as new instances of fungal species in Korea. Among these five species, P. angulare is known to able to produce cellulase and O. mirabilis as an opportunistic pathogen of human and animals.

Airborne bacteria are expected to float in the mushroom cultivation house, as it is a special environment with high humidity and high temperatures. Their concentration and diversity in the indoor air of the cultivation house could effect the health of farmers and the quality of mushrooms. To examine whether microbiota of airborne bacteria change from year to year, we measured the indoor temperature, humidity, and airborne bacterial concentration from mushroom cultivation houses located in six regions in Korea from 2020 to 2021, and isolated and identified airborne bacteria. The surveyed data were compared and the bacterial diversity of the 1st year and the 2nd year were determined. Based on the average temperature and humidity data surveyed, it can be seen that the temperature and humidity environment in the cultivation houses is such that bacteria can easily reproduce. It was observed that the temperature inside the cultivation houses tends to be higher or lower depending on the season and correlates with the temperature outside the cultivation houses. In the first year survey, 32 species of 20 genera were identified, and in the second year survey, bacteria belonging to 29 species of 22 genera were identified. Among them, the most detected species were all species belonging to the genus Bacillus. There were only three species (Bacillus altitudinis, Brevibacterium frigoritolerans, and Staphylococcus epidermidis) that were continuously isolated in common. Our results showed that the species of floating bacteria greatly vary from year to year even for the same cultivation houses.

In order for records to be preserved for a long time without physical and chemical transformation, the preservation environment of the library is very important, and environmental problems must be improved through periodic investigation on the preservation environment. Against this background, this study derived fine dust (PM10) and ultrafine dust (PM2.5) concentration data for the libraries, hallways, and workrooms of the National Archives of Korea over two years. There was a difference in the concentration of fine dust and ultrafine dust among facilities, and there was a change in the concentration depending on the month. Both fine dust and ultrafine dust concentrations were present at less than 10 μg/m³ in the libraries. In the hallways, both fine and ultrafine dust concentrations were highest in July. In the workplaces, the monthly fluctuations in the concentration of fine dust and ultrafine dust were large. And the concentration of fine dust and ultrafine dust in the workplaces were higher than those in the library and hallways. Overall, the concentration of fine dust and ultra-fine dust was measured below the maintenance standards stipulated by the Indoor Air Quality Management Act of the Ministry of Environment of Korea in all the investigated facilities. The results of this study are expected to be used as fundamental information to manage the indoor air quality of the facilities of the National Archives.

Temperature, humidity, and fungal concentration were investigated during the period from May 2019 to August 2021 to obtain information on the indoor air quality in the facilities of the National Archive of Koea, located at Seongnam, Daejeon, and Busan, respectively. The temperature and humidity of the undisinfected libraries was properly maintained for the preservation of papepr records. The airborne fungal concentration differed among the libraries, changed according to the season, and increased especially between June and August. Similarily, the concentration of airborne fungi in the hallways adjacent to the libraries also fluctuated depending on the season and showed a tendency to rise in the months when the ourside temperature rose. In general, the concentration of airborne fungi in the hallways was somewhat higher than that of the libraries. Importantly, it was identified that the concentration of airborne fungi exceeded the recommended standard (500 CFU/m3) by the Ministry of Environment of Korea only in the workroom where the classification and condition inspection of undisinfected records imported from outside were performed. Investigation of airborne fungal concentration from May 2021 to August 2021 showed that the 24 undisinfected libraries in Seongnam, Daejeon, and Busan were all good at 100 CFU/m3 or less. The results of this study are expected to be used as basic data to manage the indoor environment of facilities in the National Archives.

To obtain information on the indoor air quality of un-disinfected libraries used for paper records preservation, temperature, humidity, and bacterial concentration and species were investigated at five un-disinfected libraries from May to September in 2019 in the National Archives, Seoul (Nara Repository), Korea. Temperature and humidity of all the five un-disinfected libraries were well maintained at 18-22oC and 40-55% in compliance with the National Archives Paper Records Preservation Environment Standard. Bacterial concentration ranged from 2 CFU/m3 up to 280 CFU/m3 which were lower than the Korean indoor air quality standard value. A total of 68 bacterial species belonging to 33 genera were identified from indoor air of the five un-disinfected libraries. Among the 33 genera, Bacillus, Micrococcus, and Staphylococcus were the major genera. Only Micrococcus yunnanensis, Roseomonas mucosa, and Moraxella osloensis were commonly found among the five un-disinfected libraries. Bacterial species producing colonies with color on TSA media were present. Among the 68 species, Bacillus circulans and B. megaterium known to produce cellulases were found. There were also 17 species which have harmful effect on human health. The results of this study indicate that continuous monitoring of air borne bacteria is necessary in the un-disinfected libraries used for paper records preservation.

2016년과 2017년 서천, 장흥, 부여, 여주에 소재하는 표고 재배사에서 진균성 병원균을 모니터링 하는 과정에서 Acrodontium crateriforme, Naganishia friedmannii, Pestalotiopsis trachicarpicola, Penicillium wollemiicola, Trichoderma thailandicum 등 5 종의 국내 미기록 진균을 재배사내 실내 공기, 버섯 파리, 버섯 배지재료 등에서 분리하였다. 이들 진균은 PDA 배양하여 생장시키면서 형태적 특성 조사하고 26S rDNA, 28S rDNA, β-TUB 유전자, TEF 유전자 등을 PCR 기술로 증폭하고 그 염기서열 분석을 수행하여 동정하였다. 본 논문에서는 이들의 분류학적 위치와 위해성에 대해 보고하고자 한다.

전자상거래는 21세기를 이끌 새로운 경제 패러다임이라고들 한다. 기업들은 새로운 경영환경에 뒤쳐지지 않기 위하여 적극적으로 전자상거래를 도입하고 있는 상황이다. 그러나 기존 오프라인을 온라인화 하는 단순한 비즈니스 모델보다는 인터넷의 확장, 개방성을 치대한 활용하는 모델이 전자 상거래에 더욱 적합한 모델이 될 것이다. 이에 본 연구는 단순히 상품정보를 고객에게 제공하여 상품 구매를 바라는 수동적 운영에서 보다 적극적으로 고객과 함께 기획하고, 상품의 진열과 판매의 운영 전반에 대한 고객 참여를 유도하는 비즈니스 모델이라 할 수 있다

최근 건설현장에서 작업자의 고령화 및 숙련공 부족 현상이 중요 문제로 부각되고 있으며, 또한 최근 건설구조물이 점차 고층화, 대형화됨에 따라 부재의 형상이 복잡해지고 있다. 이러한 건설 환경의 변화에 따라 국내 및 국외에서 고유동 콘크리트에 대한 연구 및 개발이 증대되고 있다. 고유동 콘크리트는 재료분리 없이 굳지 않은 콘크리트의 유동성을 개선시켜 다짐 작업 없이 자기 충전이 가능한 콘크리트를 말한다. 하지만 고유동 콘크리트는 인장 및 휨 강도의 저하와 균열 등의 문제점을 가지고 있다. 따라서 본 연구에서는 문제점을 해결하기 위하여 고유동 콘크리트에 양마섬유를 혼입하였다. 양마섬유의 경우 천연섬유로 친환경적이며 이산화탄소의 흡수량이 많아 콘크리트 생산과정에서 배출되는 이산화탄소의 양을 상쇄시킬 수 있을 것으로 보인다. 또한, 기존 콘크리트의 섬유 보강재로 사용되었던 황마와 유사한 특성을 지녔으며 짧은 생육 기간으로 대량생산이 가능하여 경제적이다. 양마섬유는 섬유 표면이 거칠어 콘크리트와의 부착력이 뛰어나고 섬유의 인장력이 우수하여 콘크리트의 인장강도 증진과 균열 저감 효과가 있을 것으로 사료된다. 또한, 산업부산물의 일종인 플라이애쉬와 고로슬래그를 혼입하여 탄소저감 효과와 폐기물의 처리로 환경적인 측면에서 긍정적인 효과가 기대된다. 따라서 본 연구에서는 이와 같은 재료로 시멘트의 일부를 대체하여 양마섬유를 혼입한 고유동 콘크리트의 강도 특성을 알아보고자 하였다.