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pp.229-236
ATP luminescence measurements (using Relative Light Units, RLU) has been used to assess the levels of bacterial contamination on the surfaces of various materials. However, not much is known about their suitability in assessing bacterial contamination on paper surfaces. This study was conducted to evaluate the feasibility of using ATP luminometers in measuring levels of bacteria contamination on paper surfaces. The three ATP luminometers studied were Clean-Q, smart PD-30, and 3M™ Clean-Trace™ LM1 manufactured by different companies. There were some differences in RLU results among the three ATP luminometers when they were tested with different concentrations of Micrococcus luteus cell suspension. 106 - 107 cells were required in order to effectively detect Bacillus subtilis, Escherichia coli, and Micrococcus luteus on the surfaces of A4 printing sheets (100 cm2) when using the three ATP luminometers. The sizes and physical properties of surface areas varied slightly among the swabs used for the three ATP luminometers. Concentration-specific measurements (RLU) of M. luteus taken from the surfaces of six kinds of paper (fine print paper, medium print paper, ground paper, newsprint paper, practice paper, tracing paper) were possible using the smart PD-30 and LM1 ATP luminometers. ATP detection values of M. luteus varied among the six types of paper. The highest ATP detection values were found on the surfaces of tracing paper. If the RLU value is recorded at the level of 1000, this could indicate a very high bacterial contamination level of 105 to 106 CFU/4 cm2.
2. 재료 및 방법
2.1 시험 세균 균주 및 균주 배양
2.2 세균 오염 종이 시료 제조
2.3 ATP 측정
3. 결과 및 고찰
3.1 ATP luminometer 의 일반적인 검출 정도 조사
3.2 세균 농도에 따른 ATP 측정 값 비교
3.3 종이에서 세균 종과 농도에 따른 ATP 측정 값 비교
3.4 종이 재질에 따른 오염 세균의 ATP 측정
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