Ocjective : Sabaeksan has been used for the purpose of prevention and treatment of bronchial asthma and allergic asthma in korea. Methods : To investigate the biological effect of Sabaeksan, the author examined cytotoxicity and inflammatory cytokines secretion with human leukemic mast cell line, HMC-1. HMC-1 was stimulated with phorbol 12-myristate 13-acetate (PMA) and calcium ionophore A23187. Sabaeksan by itself had no effect on viability of HMC-1. Result : The effects of Sabaeksan on the secretion of tumor necrosis factor-alpha (TNF- α) and interleukin (IL)-6 from HMC-1 were evaluated with enzyme-linked immunosorbent assay. Sabaeksan (1 ㎎/㎖) inhibited PMA plus A23187-stimulated TNF-α and IL-6 secretion, by 43.86 ± 5.26%, 56.39 ± 3.65%, respectively. Sabaeksan also inhibited the NF-κB (p50/p65) expression. Conclusion : Taken together, these results suggest that Sabaeksan inhibit the production of inflammatory cytokines in HMC-1 cells through blockade of NF-κB activation.