본 연구는 날치 난소막에서 추출한 날치 난소막 추출물(Flying fish Roe Shell Extract, 이하 FRSE라 명함)의 물리화학적인 특성을 조사하기 위하여 수행되었다. 해양성 원료인 날치 난소막에서 FRSE를 제조하여 물리화학적인 특성을 분석하였다. 영양성분 조성을 분석한 결과 FRSE는 81.00%의 단백질과 9.12%의 회분, 그리고 4.48%의 수분으로 구성되어있는 것으로 나타났다. 아미노산 조성분석 결과 FRSE는 콜라겐 펩타이드의 특징인 OH-proline과 glycine이 검출되었으며, 포도당과 지방 대사에 관여하는 glutamic acid 와 aspartic acid 함량이 높게 검출되었다. 또한 열량분석 결과에서는 100g의 FRSE가 347 kcal의 열량을 지니고 있는 것으로 나타났다. FRSE의 분자량 분석 결과에서는 약 1,300 Da정도의 평균분자량 분포를 나타낸다는 것을 알 수 있었다.

In this research we extracted water-soluble collagen peptide from flatfish skin and compared it with commercially available collagen peptide extracted from Tilapia scale currently placed on the market in the aspect of physiochemical property. The physical property and nutritional components of FSCP appeared almost similarly to those of TSCP, and also in calorie, FSCP marking 3.82 Kcal showed no differences from TSCP marking 3.84 Kcal. As for forming amino acids, in aspartic acid, serine, histidine, tyrosine, methionine, FSCP had higher content than TSCP, but in OH-proline, proline and alanine FSCP had lower content than TSCP. Especially the content of essential amino acids of FSCP marked 22.74% with a higher content compared with 13.64% of TSCP. In the distribution of molecular weight FSCP with 1,000 Da showed omparatively low compared with TSCP, and in emulsion property and stability FSCP and TSCP showed similar excellent trend.

콜라겐 성분을 최대로 보호하면서 안정하게 체내로 흡수 될 수 있도록 고순도 수첨 포스 파티딜콜린과 용매사출방법을 이용하여 콜라겐 리포좀을 제조하였다. 리포좀 막의 안정성을 높이기 위해 포스파티딜콜린에 콜레스테롤을 혼합하여, 에탄올과 프로필렌글리콜 혼합용매에 용해하였으며, 이온의 안정화를 위하여 PBS Buffer를 사용하였다. 다양한 변수에 의해 제조된 콜라겐 리포좀의 특성은 동적광산란광도계(DLS), 주사현미경(SEM), 편광현미경(POM)로 분석하였다.

본 연구에서 당근에서 제조한 CG의 기능성 화장품 소재로서의 응용성을 SCP와 비교 검토하기 위하여 CG와 SCP를 적용한 각각의 기능성 크림을 제조하였다. CG와 SCP를 적용한 각각의 기능성 크림에서의 변색, 변취 및 크리밍 현상이나 응집 등의 관능검사와 pH, 점도 등의 물성 변화는 5℃, 25℃에서 매우 안정하다. 피부에서의 수분손실량 및 수분 함유량의 변화를 측정한 결과에서도 CG의 보습작용이 우수한 것으로 나타났다. 이러한 결과를 토대로 CG를 함유한 크림은 SCP를 함유한 크림과 마찬가지로 피부에 보습 효과가 우수하여 주름개선에도 큰 영향을 줄 것으로 예상된다.

Soybean lecithin liposomes composed phosphatidyl choline, phosphatidyl ethanolamine, phosphatidyl inositol and phosphatidic acid were prepared by using the previously developed supercritical reverse phase evaporation method. The effect of phospholipid composition on the formation of liposomes and physicochemical properties were examined by means of trapping efficiency measurements, transmission electron microscopy, dynamic light scattering and zeta potential measurements. The trapping efficiency of liposomes for D-(+)-glucose made of CNA-Ⅰ which contains approximately 95% phosphatidyl choline is higher than that of CNA-II and CNA-O which contain approximately 32% phosphatidyl choline. However there is no any difference between the trapping efficiency of liposomes for D-(+)-glucose made of CNA-II which has saturated hydrocarbons tails and that of liposomes made of CNA-O which has unsaturated hydrocarbon chains. The electron micrographs of liposomes made of CNA-II and CNA-O show small spherical liposomes with diameter of 0.1~0.25μm, while that of CNA-I shows large unilamellar liposomes with diameter of 0.2~1.2μm. These results clearly show that phospholipid structure of phosphatidylcholine allows an efficient preparation of large unilamellar liposomes and a high trapping efficiency for water soluble substances. Liposomes made of CNA-II and CNA-O remained well-dispersed for at least 14 days, while liposome suspension made of CNA-I separated in two phase at 14 days due to aggregation and fusion of liposomes. The dispersibility of liposomes made of CNA-I is lower than that of CNA-II and CNA-O due to the smallar zeta potential of CNA-I.

Supercritical carbondioxide is very effective in removing oils from a variety of seed matrices, devoid of any appreciable amount of phospholipid content. However, the limited solubility of phosphalipids in supercritical carbondioxide leaves behind a potentially valuable by-product in spent seed matrix. Any phospholipid extraction process from the spent matrix must maintain the structure and the quality of phospholipids and must be compatible with the end use of the seed protein meal an animal feed or for human consumption. An initial supercritical carbondioxide extraction of soybean flakes was performed at 32 MPa and 80℃ to extract the oils, leaving the phospholipids in the deflatted soybean flakes, A second step was performed on the defatted soybean flake using Xeth=0.10, Varying the pressure from 175 MPa to 70.6 MPa and temperature from 60℃ to 80℃. For all supercritical carbon dioxide/ethanol mixture extractions, a fraction rich in phospholipids was obtained. The fractions extracted from defatted soybean flakes were dried and them redissolved in chloroform before HPLC-ELSD analysis. Quantitative and qualitative analysis of phospholipids on soybean seeds, defatted soybean flake, and different extracted phospholipid fractions was carried out, to ascertain the effect of extraction pressure and temperature.