붉은사철란(Goodyera biflora)은 한국을 포함한 동아시아 지역에 분포하는 상록성 지생란으로 종자 발아율이 낮고 생장 속도가 느려 자연 상태에서의 증식이 제한적이다. 본 연구는 붉은사철란의 기내 증식 체계를 확립하고, 절편 배양 시 싸이토카이닌 처리에 따른 신초 유도 효과를 평가하며, 이를 기반으로 교육용 식물 조직배양 키트의 개발 가능성을 검토하고자 수행되었다. 무균 상태에서 배양한 줄기 마디 절편을 ½ MS 배지에 치상하고, 6-benzylaminopurine (BAP), 2-isopentenyladenine (2iP), zeatin, thidiazuron (TDZ)을 각각 0, 1, 그리고 2 mg L-1 농도로 처리하였다. 그 결과, 1 그리고 2 mg L-1 TDZ 처리구에서 가장 높은 신초 유도율(100%)과 평균 신초 수(1.3 ± 0.1)가 나타났으며, BAP와 zeatin 처리구에서도 유의한 효과가 확인되었다. 반면, 무처리구는 상대적으로 낮은 신초 유도 반응을 보였다. 이러한 결과를 바탕으로, 기내 배양묘, 원예상토, 실습 도구, 매뉴얼 및 QR 코드 연계 동영상 자료를 포함한 교육용 조직배양 키트를 설계하였다. 본 연구는 붉은사철란의 증식 및 보존을 위한 기초자료를 제공함과 동시에, 식물 조직배양 기술의 교육적 활용 가능성을 제시한다.

To increase antioxidant and antibacterial activities of seasoned soy sauce, five kinds of oriental medicinal plant(Scutellaria baicalensis (P1), Coptis japonica makino (P2), Citriunshius pericarpium (P3), Zizyphi spinosi semen (P4), Crataegus pinnatifida (P5)) and four kinds of medicinal mushrooms(Inonotus obliquus (M1), Hericium erinaceus (M2), Phellinus linteus (M3), Lentinula edodes (M4)) were added to seasoned soy sauce. Soluble solid content, pH, salinity, total polyphenol & flavonoid contents were determined. DPPH & ABTS radical scavenging activities, SOD-like activity, and antibacterial activity were analyzed. Experimental sauces showed decreased pH but significant increases of soluble solid content and salinity. Total polyphenol content was 12.76 μg GAE/g in the control. However, M1 and P1 sauce had significantly higher polyphenol contents at 352.14 and 528.25 μg GAE/g, respectively. Total flavonoids content also showed the same pattern. DPPH free radical scavenging activity was the lowest in the control at 15.75%. It was the highest at 81.80% in M1 and 68.88% in P1. ABTS free radical scavenging activity and SOD-like activity showed the same tendencies. They were higher in the experimental groups than in the control. As for the antibacterial activity analyzed by the paper-disc method, the activity increased the most in P1 and P2. In particular, P2 had the strongest antibacterial activity. Its activity against different microorganisms was in the order of Staphylococcus aureus > Bacillus cereus > Escherichia coli > Salmonella typhimurium. In conclusion, these new sauces show increased antioxidative and antioxidant activities. Therefore, they are expected to be used in various ways as a functional soy sauce.

As a fundamental analysis, this study measured and examined the mechanical property and chemical attack resistance of ceramic binder repair material at a development step through continued research. The study showed the compressive strength and bond strength of the repair material meet the standard; however, bending strength of it failed to meet the required standard. Meanwhile, chemical attack resistance displayed the excellent result.