In this study, the flexural tests were performed to investigate the flexural performance of prestressed hybrid wide flange (PHWF) beams. The main test variable was the shear stud on the top steel flange for composite action with the topping slab. All the specimens had the flexural strengths exceeding their design strengths. The test results also indicate that the PHWF beams had experienced fully composite behavior with high ductility, regardless of the existence of shear studs.