This study investigates the seismic performance of beam-column connections using Thin-Walled Steel Composite (TSC) beams and Prestressed Reinforced Concrete (PSRC) columns. TSC beams are constructed from U-shaped thin steel plates that are filled with concrete, allowing for composite action with slabs through the use of shear connectors. They are widely applied in industrial buildings due to excellent strength, stiffness, and constructability. However, slender web plates are prone to local buckling, which may compromise their performance during seismic events. To mitigate this issue, internal supports have been introduced to enhance web stability and concrete confinement. Cyclic loading tests on three specimens—with and without internal supports—demonstrated that the supports increased moment capacity, improved energy dissipation, and effectively reduced buckling. Even slender sections demonstrated performance comparable to that of compact sections. All specimens reached peak strength at a 2.44% rotation angle, with damage localized near the supports. A practical connection detail was also proposed, taking into account constructability and structural reliability. The results provide valuable guidance for the seismic design of composite systems in large-scale structures.