코이어 배지를 이용한 파프리카 재배에서 직접 정식 시 절곡 방법에 따른 정식 후 초기 생육과 수확량을 비교하였다. 절곡 방법은 I-type(절곡각도 0°), 옆으로 뉘인 L-type(절곡각도 90°), 뒤집어서 뿌리가 위로 향하도록 하는 U-type(절곡각도 180°)로 하였다. 줄기 신장 및 직경, 엽면적의 주간 평균 발달량은 정식 후 초기에 다른 처리구에 비해 영양생장이 왕성하였던 I-type에서 가장 높았다. 정식 후 46주째의 뿌리 무게도 I-type 처리구에서 다른 처리구들에 비해 약 1.3-1.7배 정도 무거웠다. 처리구별로 330주에서 생산된 수확량은 각각 약 510, 420 및 480kg으로 I-type에서 가장 높았다. 따라서 코이어 배지를 활용한 파프리카 재배에서 직접 정식 시 정식 초기의 뿌리 활착 및 세력 확보, 그리고 이후 착과 안정성에서 I-type이 적합할 것으로 판단된다.
This experiment was conducted to investigate the effect of the cysteine and glutathione on the motility index and morphology of human spermatozoa at the sperm processing in vitro. After treating the sperm with medium containing cysteine and glutathione, we measured the motility index and morphology at 0.5 h and 24 h. 1. Following the sperm culture for 0.5 h after treating the sperm with the medium containing 0, 1, 5, 10 mM cysteine, curvilinear velocity (VCL) was significantly (p<0.05) higher in control than that in all treatments. And straight-line velocity (VSL) was high at 1 mM and average path velocity (VAP) was low at 5 mM and 10 mM. But the motility (MOT) and morphology (NOM) were not different between control and all treatments. Following the sperm culture for 24 h, the MOT was significantly high in treatment groups (58.9, 74.4 and 62.3%), compared with that in control(28.7%) and the VCL was also high in treatment groups (31.4, 37.9, and 34.0 /s), compared with that in control (21.3 /s). The VSL (18.4, 21.7, and 18.9 /s) was significantly higher than control (10.7 /s) and the VAP (20.3, 24.7, and 21.4 /s) in treatments was also compared with that in control (12.6 /s). The NOM was not difference between control and treatments. 2 Following the sperm culture for 0.5 after treating the sperm with the medium containing 0, 1, 5, 10 mM glutathione, the MOT, VCL, VSL, VAP, and NOM were not different between control and treatments. Following the sperm culture for 24 h, the MOT was higher in treatment groups (82.9, 83.6, 83.4%) than in control (51.1%) and the VCL was higher in treatment groups (50.9, 51.3, and 49.4 /s) than control (34.1 /s). The VSL was also higher in treatment (17.1 /s) and the VAP was also higher in treatment groups (30.1, 32.5, and 29.7 /s) than in control (19.8 /s). The NOM was not different between control and treatments.