This study aims to enable early detection of low-concentration airborne respiratory viruses in multi-use facilities using a cyclone-based air sampler (Coriolis® m , Bertin). To achieve this, bacteriophage MS2 of Escherichia coli was aerosolized into a chamber at varying concentrations, reflecting levels observed in indoor environments. The rationale for differentiating viral concentrations was to assess field applicability and optimize sampling conditions. Sampling efficiency was maximized by adjusting sampling time, flow rate, and media volume to determine optimal detection parameters. The effectiveness of the optimized conditions was further validated through cross-validation using Influenza A and field testing. Field experiments conducted on 10 samples across five locations confirmed airborne virus detection in one of the samples (10%), demonstrating the feasibility of the cyclone-based air sampler method for airborne virus collection and detection.