In our previous study with sequenced data from DNA barcoding region of Korean Tettigonia showed that the Jeju population of T. ussuriana (JJ-Tu) more closely related to T. dolichoptera than mainland population of T. ussuriana (ML-Tu) with low genetic distance (0.87-1.05%). In mitochondrial systematics for a eukaryotic organism including orthopteran insects, sequence data from a short mitochondrial DNA fragment should be trait with caution because nuclear mitochondrial pseudogenes (numts) can be unintentionally coamplified when we use universal primers based on a PCR method. In this study, we retried their sequence analysis to avoid coamplication of numts in sequences from cox1 and cox2 genes. and scrutinized each sequence. The molecular evidences (cox1, cox2, and nad1) for Korean species suggest that JJ-Tu is more closely related to T. dolichoptera (0.76-1.23% in cox1; 1.23-1.54% in cox2; 1.01-1.35% in nad1) than ML-Tu (3.77-4.59% in cox1; 3.61-4.76% in cox2; 2.03-3.25 in nad1). The genetic distance of sequence data from cox1 between JJ-Tu and ML-Tu satisfied a requirement for species-distinction by comparing genetic distance between Tettigonia species. Moreover, JJ-Tu is a geographic population of Tettigonia with different morphological traits that is supported with formed a cluster. Although JJ-Tu closely related to T. dolichoptera with low genetic distance, we will determine its taxonomic status through integrative taxonomic study.

Phellinus linteus (PL), one of the immune-regulatory substances, is recognized to play the role in the metabolic process on inflammation and immunity. It has been traditionally used in the oriental medicine to treat inflammatory related disease. The purpose of this study was to evaluate the effects of water extracts of PL on the mesenteric lymph node lymphocytes immune function in the ICR male mice. Control mice received vehicle only. The PL treated mice were administered the respective extract by oral gavages for 4 weeks. IgE concentrations in serum and MLN lymphocytes were significantly lower in PL treated mice than in control mice. PL increased the proportion of CD4+ and CD8+ T cells in MLN lymphocytes. PL significantly decreased Th2 cytokine concentrations and mRNA expression levels in cytokine secretions. Therefore, water extracts of PL modulate inflammatory parameters through regulation of immunoglobulin production resulting from decreased Th2 cytokine secretion and mRNA expression levels and reduce pro-inflammatory cytokine secretion and mRNA expression in MLN lymphocytes.