Nanoparticles, especially those derived from plant extracts, are becoming increasingly popular as a bio-based, environmentally friendly alternative to conventional technologies. The Maui rose, a flowering plant with medicinal and therapeutic properties, is one of the most important of these materials because its extract component has antibacterial, antioxidant and anti-inflammatory biological activity. In this work, we report on synthesizing and characterizing iron oxide nanoparticles (Fe2O3) extracted from flower plants (Borago), to create persistent and environmentally friendly antibacterial agents. As part of the chemical formation process, Fe2O3 nanoparticles were extracted from specific flower plants utilizing a series of carefully regulated chemical reactions. X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and atomic force microscopy (AFM) of the samples were studied. The nanoparticles produced were analyzed using common microbiological methods and studies (EDS). The antibacterial activity of the Fe2O3 nanoparticles and their effect on a range of microorganisms were evaluated. The results demonstrated that Fe2O3 nanoparticles were successfully synthesized with a specific crystal structure and good anti-bacterial activities.