This study was performed to find the effect of Enteromorpha prolifera as a biosorbent on the removal of heavy metals such as lead, copper, zinc and cadmium from the synthetic wastewater. The biosorption experiment was conducted using biomass of dried Enteromorpha prolifera, which has caused environmental pollution issues in oceans and lakes. To find the physico-chemical characteristics and adsorption capacity, parameters such as biosorbent dosage, initial heavy metal concentration, pH value of solution, contact time which influence the effects on heavy metals removal were changed and the optimum values were found through batch test. The experimental results showed that the sequence for adsorption capacity of heavy metals by Enteromorpha prolifera was Pb2+> Cu2+> Cd2+> Zn2+. The optimum conditions of pH, contact time and dosage of biosorbent were pH 5.0, 60 min. and 0.5 g/L, respectively. As initial heavy metal concentration increased, the adsorption capacity increased up to 17.53 mg/g for Pb with 98% removal efficiency. From the adsorption thermodynamic and kinetic analysis, the biosorption pattern of Pb, Cu, Zn and Cd was well described using Freundlich and Langmuir sorption isotherm with their R2 values of 0.99 and 0.97, respectively. The sorption kinetics followed pseudosecond order kinetic models and thus supported chemical sorption rather than internal diffusion. The work clearly indicates the potential of using Enteromorpha prolifera as an excellent adsorbent for heavy metal removal in industrial wastewater.