The purpose of this study was to provide a practical guide to the use of various stretchers that rescue workers use by analyzing muscle fatigue degree associated with lifting a patient. The experiment was conducted with working male and female rescue crews. Having mixed groups of gender complete tasks such as lifting, moving, and unloading a patient using four different types of stretcher, we recorded each muscle fatigue degree in the shoulder and lower back regions of each subject. The data showed, in terms of muscle activity, differences within each group of genders and its combinations were significant in certain muscle groups depending on the types of stretcher used and body sizes of each subject, and, when it comes to Elerctor Spenae, the female subjects used more muscular strength than the males did. In regards to the muscle fatigue degree, there was no significant difference found within each group of the genders, body sizes, and their combinations. However, in the case of applying different models of stretcher, there were significantly differences in the fatigue degree.