One-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (1D SDS-PAGE) was used to determine whether it would provide improved resolving power of hordein proteins concomitant with improved identification of Korean barley cultivars and germplams. This system gave rapid and reproducible separations of hordein polypeptides. Total fourteen of clear and easily scorable subunits were identified in Korean barley cultivars and germplasms and their polymorphic constitutions could provide biochemical genetic information in progeny analysis and endosperm quality improvement in barley breeding programs. Each hordein polypeptides residing in B, C, and D hordein pattern designations were scored to prepare a cultivar catalogue of protein patterns. On the basis of this character, 7 hordein polypeptide patterns were constructed from 108 barley cultivars and experimental lines. The molecular weight of hordein subunits in Korean barley cultivars and experimental lines varied in the range of 98 to 48 kDa. In contrast, less polymorphic hordein polypeptides were found in the low protein barley lines including malting barleys than those found in Korean barley cultivars and experimental lines

Two methods of nitrogen application at the 4th leaf stage were evaluated in direct-seeding rice cultivation on dry paddy soil: one was the top dressing of nitrogen on dry paddy condition before irrigation (TNDP) for labor saving, and the other was the top dressing of nitrogen on dry paddy with mechanical rotavation of the inter-row space (TNDPIR) for increasing the efficiency of nitrogen. To evaluate these two application methods, leaf chlorophyll contents, growth characteristics and yields were compared with two conventional methods, basal application (BA) and top dressing on flooded paddy condition at the 4th leaf stage (TNFP). The SPAD value of rice in TNDP was similar with that in TNFP throughout the whole growth stage of rice. Also, there were no differences in rice growth and yield between the two methods. However, in TNDPIR, the SPAD value was the highest during tillering stage among the four application methods, and the tiller number, LAI at heading stage and the panicle number were higher than the two conventional methods.