농업 재배지 토양으로부터 auxin 생성세균 KSD16, KSD33 그리고 KSD36를 분리하였다. 분리 균주 KSD16, KSD33 그리고 KSD36 는 16S rRNA 유전자 계통분석을 통해 Arthrobacter 속으로 분류되 었다. 이들 균주는 R2A배지에 0.1% L-tryptophan를 첨가한 배지에서 28℃, 48 시간 배양한 결과, auxin 의 일종인 indole-3-acetic acid (IAA)를 204.4 mg L-1 생성하는 것으로 확인되었다. IAA 생성세균의 녹 두종자 발아에 미치는 영향을 확인한 결과, Arthrobacter 속 균주 KSD16, KSD33 그리고 KSD36 는 대조 군에 비해 뿌리길이와 발근수가 증가하였다. Arthrobacter 속 균주의 녹두 성장촉진 효과를 확인한 결과, 녹두의 발아율이 대조군보다 73.4 % 증가하는 특징을 나타내었다.

In this study, we were performed to elucidate the antioxidant and anticancer activity by leaves extracts from Acer tegmentosum (AT-L). In DPPH, ABTS radical scavenging activity, the AT-L revealed the high scavenging activity. Especially, the AT-L measured the highest ABTS radical scavenging activity, which is higher than ascorbic acid. The types of human cancer cells for evaluating the anticancer activity were colorectal cancer (SW480), prostate cancer (PC-3), breast cancer (MCF-7), pancreatic cancer (AsPC-1), lung cancer (A549) and liver cancer (HepG2). Human cancer cell viability was measured using MTT assay. Treatment of the AT-L decreased the cell viability and induced apoptosis in SW480 cells. These results suggest that extracts of the AT-L can be used as supplementary material for developing the natural antioxidant and anticancer drug for human cancer cells.

이상의 연구 결과로 먹넌출 열매 추출물은 GSK3β 의존성 Cyclin D1 단백질의 분해를 통해 대장암세포의 생육 억제와 관련이 있는 것으로 확인된다. 본 결과는 대장암의 항암제 개발을 위한 소재로 먹넌출 열매의 활용이 가능할 것으로 판단된다.

Aralia cordata (A. cordata), which belongs to Araliaceae, is a perennial herb widely distributed in East Asia. We evaluated the anti-inflammatory effect of stems (AC-S), roots (AC-R) and leaves (AC-L) extracted with 100% methanol of A. cordata and elucidated the potential signaling pathway in LPS-stimulated RAW264.7 cells. The AC-L showed a strong anti-inflammatory activity through inhibition of NO production. AC-L dose-dependently inhibited NO production by suppressing iNOS, COX-2 and IL-β expression in LPS-stimulated RAW264.7 cells. AC-L inhibited the degradation and phosphorylation of IκB-α, which donated to the inhibition of p65 nuclear accumulation and NF-κB activation. Furthermore, AC-L suppressed the phosphorylation of ERK1/2 and p38. These results suggested that AC-L may utilize anti-inflammatory activity by blocking NF-κB and MAPK signaling pathway and indicated that the AC-L can be used as a natural anti-inflammatory drugs.