Ganoderma applanatum is a medicinal mushroom belongs to Ganodermataceae of Polyporales, Basidiomycota. This study was conducted to evaluate the antioxidant, anti-inflammatory and anti-xanthine oxidase activities of G. applanatum fruiting bodies extracted with methanol. The antioxidant activities were performed on reducing power, 1,1-diphenyl-2-picrylhydrazyl free radical scavenging, and ferrous ion chelating activities. In addition to this, polyphenol and flavonoid contents were aslo analyzed. The methanol extract showed the good reducing power of 3.5 at the concentration of 4.0 mg/ml. The scavenging activity of methanol extract of G. applanatum 1,1-diphenyl-2-picrylhydrazy radicals was better than those of positive control, BHT and tocopherol. The ferrous ion chelating effect of methanol extract was moe effective than those of positive control, BHT and tocopherol. The antioxidant activities of the G. applanatum were increased with the increasing concentration of the extracts. The xanthine oxidase inhibitory activity of methanol extract of G. applanatum was good as positive control, allopurinol. The anti-inflammatory activity of mushroom extract was measured by carrageenan-induced hind paw edema of albino rat. The injection of 50 mg/kg of methanol and hot water extracts significantly reduced the carrageenan-induced paw edema compared with the positive control, indomethacin. The results indicated that fruiting bodies of G. applanatum could be used for natural medicine for anti-inflammatory and anti-xanthine oxidase.