정확한 in vitro 전사가 일어날 수 있는 진딧물의 세포추출액을 제조하였다. 전사를 직접 조절할 수 있는 단백질 인자를 규명하기 위하여 전사개시점과 그의 상류에 결합하는 DNA 결합단백질을 탐색했다. 전사개시점을 포함하는 단편 A(-194/23)에는 52kDa, 50kDa, 40kDa의 단백질들이 결합했으며 전사개시점 상류의 DNA 단편 B(-393/-263)에는 52kDa, 50kDa, 40kDa의 단백질들이 결합한 반면 DNA 단편 C(-263/-195)는 53kDa단백질만이 결합했다. 그리고 이들 DNA 결합단백질들의 DNA 결합 활성에는 양이온이 요구되었다.

High level of sequence similarity and genetic conservation within plants of same family allow us to use the informations and cDNA microarray obtained from a model plant such as Arabidopsis for better understanding of non-model plants within the same family, for example, rapeseed. Several lines of rapeseed plants with different sensitivity to cold stress were selected and the gene expression profiles under cold stress were examined using 1.6K specialized cDNA microarray. For the comparative analysis between Arabidopsis, economic plants and rapeseed, we adopted a recently developed computational method called "Gene Set Enrichment Analysis (GSEA)" which determines whether defined set of genes show statically significant and concordant differences between two biological states. Along this, five different gene sets including a network gene set based on a regulatory gene network model for early cold stress response and a co-expression gene set based on ∼ 1,500 expression data were built in this lab. With these gene sets and GSEA method, the expression data was analyzed to pinpoint the group of genes potentially responsible for the difference of stress sensitivity between two different plants. Since the plant encounters stress combinations concurrently or separated temporally and must present an integrated response to them, we built 'Cross-talk map' using ∼ 63 expression data of Arabidopsis under 9 different environmental stresses. Utilizing this cross-talk map, the significance of the identified group of genes was evaluated for their practical application to enhance stress tolerance. Currently, we identified several promising genes at a cross-talk point and are pursuing transgenic engineering to enhance the stress tolerance against more than two stress conditions.

Path planing method for an autonomous mobile robot is considered. For the practical applications, the simplified local potential field methods are applied under the constraints of the driving condition. To improve the performance, the fuzzy-approximated linear function method is also used.

Various light intensity and light quality were treated to oat seedlings to investigate the effect of light on the chlorophyll accumulation and the formation of chlorophyll-protein complexes. The increase of total chlorophyll accumulation and Chl (chlorophyll) a/b ratio was promoted under H (high intensity) white light during oat chloroplast development when compared to L (low intensity) white light. Also H white right was more effective in the formation of chlorophyll-protein complexes associated with PSI, CCI and CCII than L white light. The seedlings grown in various light quality caused little changes in total chlorophyll and Chl a/b ratio when compared to those grown in L white light. The assembly of LHCII trimer was more affected by L white light treatment in the formation of-chlorophyll-protein complexes than red light treatment. The effect of blue light on the relative composition of chlorophyll-protein complexes was similar to that of L white light. Particualrly, blue light was more effective in the synthesis of LHCII monomer than the other hot quality at the early stage of greening. When compared to red light, blue light was more effective in the formation of LHCII monomer. These results suggest that light intensity is more effective in the increase of chlorophyll accumulation and Chl a/b ratio than light quality, and light quality may be an important factor for the regulation of the organization in the chlorophyll-protein complexes during greening.