This study was conducted to provide basic data for high-throughput screening (HTS) system construction based on phenomics. Rice (Oryza sativa cv. Chucheongbyeo) seedlings in vegetative growth stage were grown in the glass house and treated with 0, 3.75, 7.5, 15, and 30% (w/v) of polyethylene glycol (PEG) to give osmotic stress. Three days after PEG treatment, hyper-spectral reflectance images were obtained and analyzed after removing background image in several steps. The reflectance of rice seedlings treated with 15 and 30% of PEG solutions were significantly different at 680 nm, where differences in the chlorophyll reflectance spectrum and visual symptoms were not observed. These results thus indicate that hyper-spectral reflectance observed at 680 nm can be used to screen drought tolerant rice lines. A HTS system equipped with this hyper-spectral reflectance system may play an important role of future rice breeding program.

This study was conducted to investigate plant body temperature response of soybean (Glycine max) to saline stress. Two-weeks-old seedlings of soybean in V1 growth stage were treated with 0, 10, 20, 40, 80 and 160 mM of NaCl for salt stress. Thermal images acquired using Flir T-420 (US) were obtained at 4 days after treatment. Soybean leaf temperature increased with increasing NaCl concentration, resulting in significant positive correlation between soybean leaf temperature and stress intensity (P < 0.01). Leaf temperature of soybean was significantly different at 160 mM of NaCl, where no visual symptom was observed. Therefore, soybean leaf temperature can be used for evaluating the response of soybean to salt stress as a non-destructive and phenomic parameter. Non-destructive diagnosis of soybean leaf temperature may be a key parameter in a high throughput screening (HTS) system in breeding program for salt stress tolerance soybean cultivars.