The aim of this study was to identify the non-Aggregatibacter actinomycetemcomitans bacteria grown on the tryptic soy-serum-bacitracin-vancomycin (TSBV) medium, an A. actinomycetemcomitans selective medium. A total of 82 unidentified bacterial isolates from the oral cavities of a Korean population were kindly provide by the Korean Collection for Oral Microbiology. All the clinical isolates were grown on TSBV medium and bacterial DNA purified from each isolate was subjected to PCR with universal primers specific for bacterial 16S rRNA genes (16S rDNAs) sequence. The each bacterial 16S rDNA was amplified by PCR and the nucleotide sequences of it was determined by the dideoxynucleotide chain termination method. They were identified by 16S rDNA sequence comparison method at the specie-level. The data showed that Neisseria spp. (42 strains), Fusobacterium spp. (10 strains), Capnocytophaga spp. (8 strains), Propionibacterium acnes (5 strains), Aggregatibacter aprophilus (4 strains), Campylobacter spp. (5 strains), Veillonella dispar (3 strains), Streptococcus sp. (1 strain), Haemophilus parainfluenzae (1 strain), Leptotrichia wadei (1 strain), Morococcus sp./Neisseria sp. (1 strain), and Staphylococcus sp. (1 strain) were identified. These results could be used to develop a new A. actinomycetemcomitans-selective medium which is more effective than the TSBV medium in future studies.

This paper describes the initial operations and preliminary results of the Instrument for the study of Stable/Storm-time Space (ISSS) onboard the microsatellite Next Generation Small Satellite-1 (NEXTSat-1), which was launched on December 4, 2018 into a sun-synchronous orbit at an altitude of 575 km with an orbital inclination angle of 97.7°. The spacecraft and the instruments have been working normally, and the results from the observations are in agreement with those from other satellites. Nevertheless, improvement in both the spacecraft/instrument operation and the analysis is suggested to produce more fruitful scientific results from the satellite operations. It is expected that the ISSS observations will become the main mission of the NEXTSat-1 at the end of 2020, when the technological experiments and astronomical observations terminate after two years of operation.