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        1.
        2023.09 KCI 등재 구독 인증기관 무료, 개인회원 유료
        Narcissus (Narcissus spp.) bulbs were used to investigate contagious viral retention in the dormant stage. The amplified polymerase chain reaction (PCR) bands were screened using universal potyvirus primers and sequenced to identify specific viruses. To identify individual viruses, primers for specific viruses were designed, and reverse transcription (RT)-PCR was performed. The PCR fragments were sequenced and compared by phylogenetic analysis. Three potyviruses, cyrtanthus elatus virus A (CyEVA), narcissus yellow stripe virus (NYSV), and narcissus late season yellows virus (NLSYV) were singly or doubly detected in 18 samples from six cultivars of the 38 samples representing eight cultivars that were tested. Based on RT-PCR using specific primers, CyEVA was detected in the split corona daffodil cv. Orangery. NLSYV and NYSV frequently occurred as co-infections with each other in the double daffodil cv. Tahiti, and the N. cyclamineus daffodil cv. Tete-a-tete and cv. Tete Boucle. Results has shown that at least three potyviruses are present in imported Narcissus bulbs.
        4,000원