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        2010.12 KCI 등재 구독 인증기관 무료, 개인회원 유료
        Five different enrichment methods were studied to find an optimal method to recover Yersinia enterocolitica from swine feed samples. When the recovery of Y. enterocolitica GER-C (serotype O:3) strain was studied at 1000 CFU/g feed, phosphate-buffered saline (PBS) enrichment at 4℃ and PBS plus sorbitol and bile salts (PSB)enrichment at 4℃ and 21℃ were not effective (< 22%). In contrast, both irgasan-ticarcillin-potassium chlorate (ITC)and tryptic soy broth plus polymyxin B sulfate and novobiocin (TSBPN) enrichment methods showed a full recovery (100%) at 100-1000 CFU/g feed. At 10 CFU/g feed, both ITC and TSBPN methods still recovered the strain (> 50%). In recovery of ATCC 9610 (serotype O:8) strain, TSBPN method was more sensitive than any other methods (P < 0.05) at 1000 CFU/g feed. Using TSBPN method, the strain was still recovered at 100 CFU/g feed, but not at 10CFU/g feed. With its sensitivity and relatively simple recipe, TSBPN was most desirable method to recover Y. enterocolitica from swine feed samples.
        4,000원