In this study we attempted to analyze the change of protein and mycelial activity during low temperature storage progressed. At first, esterase and peroxidase isozymes were detected at third and fourth subculture in Pleurotus ostreatus and peroxidase isozymes in Pleurotus eryngii were detected at first and second subculture but esterase isozymes showed almost same band patterns subculture advanced. The decolorization ratio of media color including indicator were decreased according to subculture advanced in P. ostreatus and P. eryngii. However there were not significantly difference of mycelial growth, fruit body yield, esterase isozyme band pattern and laccase activity of P. eryngii according to storage periods at low temperature. Therefore, it was possible to store sawdust spawn of P. eryngii at 4℃ for 120days.

This study was carried out to set up ventilation coefficient for high quality fruit body. We made the ventilation apparatus for ventilation characteristics of oyster mushroom. The pattern of growth of oyster mushroom for 4 stage, 1stage of the period of primordial induction and 3 stage of the period of fruit body formation, was investigated. That was favorable configuration of the fruit body at the carbon dioxide concentration under 1,500ppm. The higher was ventilation coefficient, the more carbon dioxide injury generated, and the ventilation coefficient of the late growth was low. In the case of Chunchu 2-ho, those quality were high when ventilation coefficient as the lapse of growth stage set up 8, 6, 4, 4, and Suhan 2-ho was 6, 6, 4, 2. Base on these results, if the optimum ventilation coefficient set up, the cultivator will cultivate good production.

In this study we attempted to analyze the change of protein and mycelial activity during low temperature storage progressed. At first, esterase and peroxidase isozymes were detected at third and fourth subculture in Pleurotus ostreatus and peroxidase isozymes in Pleurotus eryngii were detected at first and second subculture but esterase isozymes showed almost same band patterns subculture advanced. The decolorization ratio of media color including indicator were decreased according to subculture advanced in P. ostreatus and P. eryngii. However there were not significantly difference of mycelial growth, fruit body yield, esterase isozyme band pattern and laccase activity of P. eryngii according to storage periods at low temperature. Therefore, it was possible to store sawdust spawn of P. eryngii at 4℃ for 120days.

This study was carried out to set up ventilation coefficient for high quality fruit body. We made the ventilation apparatus for ventilation characteristics of oyster mushroom. The pattern of growth of oyster mushroom for 4 stage, 1stage of the period of primordial induction and 3 stage of the period of fruit body formation, was investigated. That was favorable configuration of the fruit body at the carbon dioxide concentration under 1,500ppm. The higher was ventilation coefficient, the more carbon dioxide injury generated, and the ventilation coefficient of the late growth was low. In the case of Chunchu 2-ho, those quality were high when ventilation coefficient as the lapse of growth stage set up 8, 6, 4, 4, and Suhan 2-ho was 6, 6, 4, 2. Base on these results, if the optimum ventilation coefficient set up, the cultivator will cultivate good production.