We aim to elucidate suitable color of light during development of fruit body in Pleurotus eryngii. The four color of LED(Light Emitting Diode), blue LED(475nm), green LED(525nm), yellowed LED(590nm), and red LED(660nm), were irradiated for formation of fruit body after mycelia growth. First, as effect of color of LED at all growth stage, the morphological properties of fruit body in fluorescent lamp and blue LED irradiation were showed thicker and longer pileus than those in other color LED irradiation. The commercial yields in blue and green LED treatment were similar to control(Fluorescent lamp). Second, as effect of color of LED by light irradiation time, we could obtain the highest commercial yields at the green LED continuous radiation, 1/1 and 1/2(lighting/lighting-out, hours). And we could obtain the highest number of available stipes and biological efficiency at the 1/1(lighting/lighting-out, hours). The ergosterol was the highest under the green LED continuous radiation.

We found about cultivation environment conditions of N. lepideus. For the high quality of N. lepideus, lights-out was useful. In the lights-out, the diameter of pileus of N. lepideus was smaller, and the length and the thickness of stipes were longer and larger than in that of lighting. For the efficient cultivation of N. lepideus, temperature of mycelial growth and development of the fruit body were suitable 20～23℃ and 20℃, respectively. In addition, we found higher yield and better quality of N. lepideus at 1,500～2,000ppm of CO2 concentration during the development of fruit body.

This study was carried out to determine the optimum dietary supplementation level of oyster mushroom in cherry salmon (Oncorhynchus masou masou). Juvenile cherry salmon averaging 5.0±0.5g (mean ± SD) were fed one of the five experimental diets containing 0, 3.5, 7.0, 10.5 and 14.0% oyster mushroom (D0, D3.5, D7.0, D10.5 and D14.0) for 12weeks. Increasing of dietary beta-glucan content were observed at a high dietary oyster mushroom powder. After the feeding trial, average weight gain (WG) of fish fed D0, D3.5 and D7.0 diets were significantly higher than those of fish fed D10.5 and D14.0 diets (P < 0.05), however there were no significant differences in WG among fish fed D0, D3.5 and D7.0 diets (P > 0.05). Average feed efficiency (FE) of fish fed D0, D3.5 and D7.0 diets were significantly higher than those of fish fed D10.5 and D14.0 diets (P < 0.05), however there were no significant differences in FE among fish fed D0, D3.5 and D7.0 diets (P > 0.05). Average hepatosomatic index (HSI) of fish fed D0, D3.5, D7.0 and D10.5 diets were significantly higher than those of fish fed D14.0 diets (P < 0.05), however there were no significant differences in HSI among fish fed D0, D3.5, D7.0 and D10.5 diets (P > 0.05). Therefore, these results indicated that the optimum dietary supplementation level could be greater than 3.5%, but less than 7.0% in juvenile cherry salmon under our experimental conditions. And additional research on the immune response will be necessary to carry out.

This study was carried out to elucidate suitable substrate making of Lentinus lepideus for an artificial cultivation. We could obtain the highest yield of fruit body and shortest period of cultivation in the case of pine sawdust and deffated corn flour mixing substrate. And suitable mixing ratio of main substrate and additives was selected 90:10(v/v). Consequently, it is necessary to elucidate suitable environmental conditions in fruit body step for the good quality and stable production of this mushroom.

This study was carried out to set up ventilation coefficient for high quality fruit body. We made the ventilation apparatus for ventilation characteristics of oyster mushroom. The pattern of growth of oyster mushroom for 4 stage, 1stage of the period of primordial induction and 3 stage of the period of fruit body formation, was investigated. That was favorable configuration of the fruit body at the carbon dioxide concentration under 1,500ppm. The higher was ventilation coefficient, the more carbon dioxide injury generated, and the ventilation coefficient of the late growth was low. In the case of Chunchu 2-ho, those quality were high when ventilation coefficient as the lapse of growth stage set up 8, 6, 4, 4, and Suhan 2-ho was 6, 6, 4, 2. Base on these results, if the optimum ventilation coefficient set up, the cultivator will cultivate good production.

In this study we attempted to analyze the change of protein and mycelial activity during low temperature storage progressed. At first, esterase and peroxidase isozymes were detected at third and fourth subculture in Pleurotus ostreatus and peroxidase isozymes in Pleurotus eryngii were detected at first and second subculture but esterase isozymes showed almost same band patterns subculture advanced. The decolorization ratio of media color including indicator were decreased according to subculture advanced in P. ostreatus and P. eryngii. However there were not significantly difference of mycelial growth, fruit body yield, esterase isozyme band pattern and laccase activity of P. eryngii according to storage periods at low temperature. Therefore, it was possible to store sawdust spawn of P. eryngii at 4℃ for 120days.

This study was carried out to set up ventilation coefficient for high quality fruit body. We made the ventilation apparatus for ventilation characteristics of oyster mushroom. The pattern of growth of oyster mushroom for 4 stage, 1stage of the period of primordial induction and 3 stage of the period of fruit body formation, was investigated. That was favorable configuration of the fruit body at the carbon dioxide concentration under 1,500ppm. The higher was ventilation coefficient, the more carbon dioxide injury generated, and the ventilation coefficient of the late growth was low. In the case of Chunchu 2-ho, those quality were high when ventilation coefficient as the lapse of growth stage set up 8, 6, 4, 4, and Suhan 2-ho was 6, 6, 4, 2. Base on these results, if the optimum ventilation coefficient set up, the cultivator will cultivate good production.

In this study we attempted to analyze the change of protein and mycelial activity during low temperature storage progressed. At first, esterase and peroxidase isozymes were detected at third and fourth subculture in Pleurotus ostreatus and peroxidase isozymes in Pleurotus eryngii were detected at first and second subculture but esterase isozymes showed almost same band patterns subculture advanced. The decolorization ratio of media color including indicator were decreased according to subculture advanced in P. ostreatus and P. eryngii. However there were not significantly difference of mycelial growth, fruit body yield, esterase isozyme band pattern and laccase activity of P. eryngii according to storage periods at low temperature. Therefore, it was possible to store sawdust spawn of P. eryngii at 4℃ for 120days.

Root colonization by a rhizobacterium, Pseudomonas chlororaphis O6, elicited induced systemic resistance (ISR) in the leaves of cucumber plants against fungal and bacterial pathogens. To understand the role of unique genes during strain O6-mediated ISR, a suppressive subtractive hybridization method was undertaken and led to isolation of twenty-five distinct genes. The transcriptional levels of all the genes showed an increase much earlier under O6 treatment than in water control plants only after challenge with pathogen, while no difference detected on the plants without pathogen challenge. This suggests that O6-mediated ISR is associated with the priming phenomenon, an enhanced capacity for the rapid and effective activation of cellular defense responses after challenge inoculation.