Sperm chromatin integrity is essential for successful fertilization and development of an embryo. Reported here is a quantification of DNA fragments which is intimately associated with reproductive potential to provide one of criteria for sperm chromatin integrity. Three sperm populations were considered: CONTROL (no treatment), UV irradiation (48mW/, 1h) and (oxidative stress induced by hydrogen peroxide, 10 mM, 50 mM and 100 mM). DNA fragments in boar sperm were evaluated by using ligation-mediated quantitative real-time polymerase chain reaction (LM-qPCR) assay, which relies on real-time qPCR to provide a measure of blunt 5' phosphorylated double strand breaks in genomic DNA. The results in agarose gel electrophoresis showed no significant DNA fragmentation and no dose-dependent response to . However, the remarkable difference in shape and position was observed in melting curve of LM-qPCR. This result supported that the melting curve analysis of LM-qPCR presented here, could be more sensitive and accurate than previous DNA fragmentation assay method.

This study was conducted to investigate the growth characteristics and functional materials of baby vegetables as affected by different LEDs and luminous intensity at Anseongsi, Gyeonggi Province, in 2014. Test crops were beet, chicory, spinach, red leaf lettuce, crown daisy and red mustard purchased from the seed company of Dongbu Hannong and Jinheung. Growth characteristics were measured and the content of functional materials was analyzed 40 days after seeding at plug plate. Treatment of Red+Blue (4:1) at 150 µmol m-2 s-1 luminous intensity showed the highest number of leaves in five baby vegetables of beet, chicory, red leaf lettuce, crown daisy and red mustard. The highest shoot length of chicory, spinach, red leaf lettuce, crown daisy and red mustard was obtained from the treatment of Red+Blue (4:1) at 150 µmol m-2 s-1 luminous intensity. Fresh weight and dry weight of all six baby vegetables were the highest in treatment of Red+Blue (4:1) at 150 µmol m-2 s-1 luminous intensity. Content of chlorophyll a and chlorophyll b of spinach, red leaf lettuce and red mustard showed the highest in Fluorescent lamp at 150 µmol m-2 s-1 luminous intensity whereas other crops did not show definite trend under different LEDs lights and luminous intensity. The highest total content of anthocyanins and polyphenol were obtained from the treatment of Red+Blue (4:1) at 150 µmol m-2 s-1 luminous intensity in all six baby vegetables. Free radical scavenging activity was highest in all six vegetable crops at 150 µmol m-2 s-1 luminous intensity, but it was not different significantly between LEDs.As a result, the growth and the content of functional material of baby vegetables are generally to be increased in Red+Blue (4:1) at 150 µmol m-2 s-1 luminous intensity. Mixed light of Red+Blue is thought to give good effect on the growth and the content of functional material in baby vegetable crops. Because there are many differences in regard of LED lights, crop varieties, cultivation and experi-mental methods in their impact on the growth and functional materials of baby vegetables among researchers, it is considered that a more precise studies are needed for the crop responses to LED light and luminous intensity.