Western drywood termite, Incisitermes minor, is distributed southwestern USA and northern Mexico. Invasions had been reported to Canada, China, Hawaii and Japan. Incisitermes minor may be introduced through wooden structures. In California and Arizona, it caused economic damage of about $250 million. In September 2023, it was discovered in a house in Changwon-city, Korea and a joint investigation was conducted by APQA and other government agencies. The 16S rRNA sequence was used in the molecular epidemiological investigation to trace the origin of the invasion. Five individuals found in Changwon and 18 foreign sequences collected by NCBI were compared. A total of 462bp of base sequences were compared, and the genetic distance was observed to range from a minimum of 0.000 to a maximum of 0.1791. They were most genetically similar to the California individual in the United States.

Drywood termite (Cryptotermes domesticus), native to South East Asia, is distributed in India, China, Taiwan, Australia and so on. In China, it is considered an economically important pest causing damage to wood. It lives in dry wood environments such as furniture, buildings, and structural timber. It is difficult to find drywood termite within a structure but it can be detected by the presence of alate flight and small egg-like pellets of excreta. It was found in a house in Seoul, Korea in July 2023 and was investigated joint by APQA, National Institute of Ecology, National Institute Biological Resources, National Institute of Forest Science, Cultural Heritage Administration and Gyeongsang National University. The COII barcode region was analized with PCR method and compared with the sequences of all C. domesticus registered in NCBI 557bp of base sequence. Genetic difference ranged from a minimum of 0.0072 to a maximum of 0.0557. Among these, the individual with the minimum genetic distance was from Guangdong Province, China.

개나무좀과 (Bostrichidae)에 속하는 Lyctoxylon dentatum (Pascoe, 1866)(가슴각진개나무좀; 신칭), Melalgus batillus (Lesne, 1902)(큰머 리개나무좀; 신칭)을 채집하여 한반도에서 처음으로 보고한다. 본 연구는 이들에 대한 간단한 진단형질과 종 정보 및 사진자료를 제공하고자 한다.

When shooting an object with a microscope, the size of the object is large, so it may not be captured on one screen, or the results may not be good due to dust. It is intended to provide a high-resolution image photographing method and correction method using Photoshop 5.0 program. In particular, when the object is large, a method of synthesizing after split photographing when it is not visible on one screen during micrographing will be provided. In addition, when the background is dark, a background color correction method and a color correction method after imaging are also provided. The photographing method and correction method are presented based on Lepidoptera, Diptera, and Coleoptera. The high-resolution images were taken using Nikon D500 16-80mm VR Kit or Leica M205C, Dhyana400DC.

코드린나방은 사과, 배, 복숭아 등의 과실류를 비롯한 다양한 작물의 해충으로써, 국내에서는 검역 대상으로 금지해충에 속해있다. 코드린나방이 속한 잎말이나방의 유충은 과실 속으로 파고 들어가 살기 때문에 살충제로 방제하기가 어려운 해충이다. 따라서 철저한 수입식물 검역과 예찰을 통해 국내로의 유입을 막는 것이 최선이다. 현재 잎말이나방류에 대한 예찰용 페로몬트랩이 사용되고 있지만 개별 종에 대한 표적 예찰은 이뤄지지 않고 있다. 본 연구에서는 코드린나방의 성페로몬을 이용한 유인제 개발을 위한 기초연구로써, 현재 상용화되어 미국, 유럽, 중국 등에서 사용하고 있는 다양한 유인제의 조성성분을 분석하였다. 전세계적에서 대표적으로 사용되는 4종류의 코드린나방 루어를 GC-MS/MS를 이용하여 물질의 종류와 구성비율을 분석하였다. 본 연구의 결과는 향후 코드린나방의 최적 예찰을 위한 성페로몬 유인제 선발의 기초자료로 활용될 예정이다.

본 연구는 국내분포가 기록된 해충, 네눈쑥가지나방의 발생조사를 위해 실시되었다. 네눈쑥가지나방의 국내분포 발생조사를 실시하기 전 국내에 보고된 문헌과 각 연구기관의 네눈쑥가지나방 DB 및 표본을 확인하였다. 국내 발간된 문헌을 확인한 결과 한국경제곤충, 국립생물자원관에서 발간한 국가생물종목록집 및 다양한 논문과 연구보고서에서 네눈쑥가지나방의 기록을 확인할 수 있었다. DB 및 표본 확인결과 농촌진흥청에서 1924년부터 1996년까지 38건의 DB를 확인하였고, 온난화대응연구소에서 68점의 표본을 확인하였다. 또한 농림축산검역본부 제주지역본부에 서 네눈쑥가지나방의 DB와 186점의 표본을 확인하였다. 국립수목원에서는 1979년부터 2011년까지 183건의 DB를 확인하였고, 국립생물자원관에서는 408건의 DB를 확인하였다. 조사결과를 토대로 네눈쑥가지나방은 우리나라 전 지역에 분포하는 것으로 확인되었다.

여행객 휴대 수입금지식물에서 발생하는 금지해충(과실파리류, 코드린 나방 등)의 유입 가능성이 높아짐에 따라 지속적인 모니터링을 통하여 품목별, 국가별로 검출되는 해충의 정보 축적이 필요하며, 검역해충 종 동정을 위하여 표본 확보 및 분류학적 정보가 필요하다. 2018년 1월부터 12월까지 인천공항 및 인천항으로 반입되어 압수한 휴대식물 중 사과, 배, 복숭아에서 검출되는 해충을 조사하였다. 그 결과 형태학적 동정 및 DNA 바코딩 분석을 통하여 Carposina sasakii (복숭아심식나방), Grapholta molesta (복숭아순나방), Grapholita dimorpha (복숭아순나방붙이) 등이 검출되었지만 우려하는 코드린나방은 검출되지 않았다. 본 연구결과는 휴대 반입식물에서 검출되는 해충 종 구성에 대한 검역자료로 활용될 수 있을 것으로 판단된다.

In the 21st century, the risk of the exotic pest being invaded is increasing due to the rise of trading activities. Oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae), has been considered to very destructive invader as it is highly intrusive and has a wide host range. In this study, 2,299 samples were collected from 88 locations in 12 countries by quarantine and investigation. Among them, 608 individuals (including B. correcta) were used for COI DNA barcoding analysis based on Neighbor Joining method with P-distance model. Population genetics analysis was conducted for 510 individuals selected from 47 locations of 12 Southeast Asian countries using 15 microsatellite loci. The barcoding analysis resulted in that any clade was not clustered according to a geographical isolation but indicated geographically mixed populations. Population genetics analysis showed shared genetic structure between neighboring countries across borders. Genetic structure of most Korean quarantine groups was more similar to that of Taiwan, China and Thailand in the order of appearance than other countries.

The fall armyworm (FAW), Spodoptera frugiperda J.E. Smith is a noctuid moth endemic throughout the Western Hemisphere that has recently become widespread in sub-Saharan Africa. In Asia, FAW was firstly reported at corn fields in India, SriLanka, Bangladeshi, Miyanmar and Thailand in 2018. In January 2019, FAW was also found in Yunnan province of China. In March 2019, the larvae which could be tentatively identified as FAW were caught at a corn field of Plant Protection Center of Lao PDR, which is located in Vientiane, Laos. Species identification was confirmed by DNA barcoding using the COⅠ segment of the four larvae, which were found to be the haplotype of rice strain (COⅠ-RS). The host strain identity was additionally analysed as a Tpi-C (C-strain allele) by Triosephosphate isomerase gene (Tpi) segment located on the Z sex chromosome. The result shows that the FAW specimens in Lao is the subpopulation of COⅠ-RS/Tpi-C (COⅠ and Tpi haplotype combination). It was reported that COⅠ-CS/Tpi-C were more frequently observed than COⅠ-RS/Tpi-C in Western Hemisphere and Western Africa, but COⅠ-RS/Tpi-C were more frequently observed in Eastern Africa. It can be supposed that the subpopulation of COⅠ-RS/Tpi-C in Lao is one of the subpopulations which have migrated into the Indochinese peninsula from Eastern Africa, with more detailed analysis for more diverse nationwide specimens left.